{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:45:28Z","timestamp":1780717528108,"version":"3.54.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T00:00:00Z","timestamp":1323388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Microglia\u2010mediated inflammation in the central nervous system is a hallmark of the pathogenesis of several neurodegenerative diseases including Alzheimer's disease. Microglial cells activation follows the deposition of amyloid \u03b2 fibrils and it is generally considered a triggering factor in the early steps of the onset of Alzheimer's disease. Although the initial engagement of microglia seems to play a neuroprotective role, many lines of evidence indicate that a persistent activation with the production of proinflammatory molecules contributes to dismantle neuronal activity and to induce neuronal loss occurring in neurodegenerative diseases. To date, limited proteomic data are available on activated microglial cells in response to extracellular amyloidogenic peptides. In this study, murine microglial cells have been employed to investigate the effects of amyloid \u03b2 peptides in triggering microglial activation. The response was monitored at the proteome level through a two\u2010dimensional gel electrophoresis\u2010based approach. Results show only a limited number of differentially expressed proteins, among these a more acidic species of the cytosolic actin, and the 14\u20103\u20103\u03b5 protein, found significantly upregulated in A\u03b2\u2010activated cells. 14\u20103\u20103\u03b5 belongs to a regulatory protein family involved in important cellular processes, including those leading to neurodegenerative diseases, and thus its increased expression suggests a role of this protein in tuning microglia activation.<\/jats:p>","DOI":"10.1002\/pmic.201100113","type":"journal-article","created":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T05:57:52Z","timestamp":1320731872000},"page":"124-134","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["14\u20103\u20103\u03b5 marks the amyloid\u2010stimulated microglia long\u2010term activation"],"prefix":"10.1002","volume":"12","author":[{"given":"Laura","family":"Di Francesco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Correani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinzia","family":"Fabrizi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenzo","family":"Fumagalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Mazzanti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Maras","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Eugenia","family":"Schinin\u00e0","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69113-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132814"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/325733a0"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700311"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1566067"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05290"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132528"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2010.17"},{"key":"e_1_2_6_10_2","first-page":"307","article-title":"Microglial response to amyloid plaques in APPsw transgenic mice","volume":"152","author":"Frautschy S. A.","year":"1998","journal-title":"Am. J. Pathol."},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004010050664"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1159\/000113699"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(00)00124-X"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(03)00253-4"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.05.047"},{"key":"e_1_2_6_16_2","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1074\/mcp.M500114-MCP200","article-title":"Microglial activation induced by neurodegeneration: a proteomic analysis","volume":"4","author":"Zhou Y.","year":"2005","journal-title":"Mol. Cell. Proteomics"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2007.04568.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(90)90073-V"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.14573\/altex.2009.2.83"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja010452r"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(89)90397-9"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900085"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol.2009.09.005"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.02.048"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(03)00447-6"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806192105"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-4580(01)00306-2"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306183200"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0705377"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00221-006-0819-6"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.20870"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1471-4159.1997.69062285.x"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-010-9528-6"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2007.10.007"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(98)00280-8"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1159\/000080469"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900195"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5572-08.2009"},{"key":"e_1_2_6_39_2","first-page":"44","article-title":"Effects of F\/G\u2010actin ratio and actin turn\u2010over rate on NADPH oxidase activity in microglia","volume":"11","author":"Rasmussen I.","year":"2010","journal-title":"Biomed. Chromatogr. Immunol."},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2006.03.005"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2008.10.003"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrn1197"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/ageing\/afn094"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.55.6.811"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr9001614"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.22528"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63322-6"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(96)12598-2"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9003777"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201100113","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201100113","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:27:28Z","timestamp":1761056848000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201100113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,12,9]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1002\/pmic.201100113"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201100113","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,12,9]]},"assertion":[{"value":"2011-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-10-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-12-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}