{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:59:06Z","timestamp":1791093546451,"version":"4.1.0"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T00:00:00Z","timestamp":1335398400000},"content-version":"vor","delay-in-days":56,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>The versatility of the surface of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia<\/jats:italic>, the causative agent of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme borreliosis, is very important in host\u2013pathogen interactions allowing bacteria to survive in ticks and to persist in a mammalian environment. To identify the surface proteome of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia<\/jats:italic>, we have performed a large comparative proteomic analysis on the three most important pathogenic <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia<\/jats:italic> species, namely <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>. burgdorferi<\/jats:italic> (strain <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>31), <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>. afzelii<\/jats:italic> (strain <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>78), and <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>. garinii<\/jats:italic> (strain <jats:styled-content style=\"fixed-case\">PB<\/jats:styled-content>i). Isolation of membrane proteins was performed by using three different approaches: (i) a detergent\u2010based fractionation of outer membrane proteins; (ii) a trypsin\u2010based partial shedding of outer cell surface proteins; (iii) biotinylation of membrane proteins and preparation of the biotin\u2010labelled fraction using streptavidin. Proteins derived from the detergent\u2010based fractionation were further sub\u2010fractionated by heparin affinity chromatography since heparin\u2010like molecules play an important role for microbial entry into human cells. All isolated proteins were analysed using either a gel\u2010based liquid chromatography (<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">MS\/MS<\/jats:styled-content> technique or by two\u2010dimensional (2<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>)\u2010<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS\/MS<\/jats:styled-content> resulting in the identification of 286 unique proteins. Ninety seven of these were found in all three <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia<\/jats:italic> species, representing potential targets for a broad coverage vaccine for the prevention of <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>yme borreliosis caused by the different <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>orrelia<\/jats:italic> species.<\/jats:p>","DOI":"10.1002\/pmic.201100211","type":"journal-article","created":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T16:43:52Z","timestamp":1335458632000},"page":"845-858","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Comparative membrane proteome analysis of three <i><scp>B<\/scp>orrelia<\/i> species"],"prefix":"10.1002","volume":"12","author":[{"given":"Bernd","family":"Gesslbauer","sequence":"first","affiliation":[{"name":"ProtAffin Biotechnologie AG  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albina","family":"Poljak","sequence":"additional","affiliation":[{"name":"Intercell AG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Handwerker","sequence":"additional","affiliation":[{"name":"ProtAffin Biotechnologie AG  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Sch\u00fcler","sequence":"additional","affiliation":[{"name":"Intercell AG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Schwendenwein","sequence":"additional","affiliation":[{"name":"ProtAffin Biotechnologie AG  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinna","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Sciences University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Urban","family":"Lundberg","sequence":"additional","affiliation":[{"name":"Intercell AG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Meinke","sequence":"additional","affiliation":[{"name":"Intercell AG  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas J.","family":"Kungl","sequence":"additional","affiliation":[{"name":"ProtAffin Biotechnologie AG  Graz Austria"},{"name":"Institute of Pharmaceutical Sciences University of Graz  Graz Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,4,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/17.4.708"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"2679","DOI":"10.1128\/jcm.33.10.2679-2685.1995","article-title":"Analysis and comparison of plasmid profiles of \n                     Borrelia burgdorferi sensu lato strains","volume":"33","author":"Xu Y.","year":"1995","journal-title":"J. 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