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There is no published report on <jats:italic>L. donovani<\/jats:italic> genome sequence available till date, although the genome sequences of three related <jats:italic>Leishmania<\/jats:italic> species are already available. Thus, we took a proteogenomic approach to identify proteins from two different life stages of <jats:italic>L. donovani<\/jats:italic>. From our analysis of the promastigote (insect) and amastigote (human) stages of <jats:italic>L. donovani<\/jats:italic>, we identified a total of 22322 unique peptides from a homology\u2010based search against proteins from three <jats:italic>Leishmania<\/jats:italic> species. These peptides were assigned to 3711 proteins in <jats:italic>L. infantum,<\/jats:italic> 3287 proteins in <jats:italic>L. major<\/jats:italic>, and 2433 proteins in <jats:italic>L. braziliensis<\/jats:italic>. Of the 3711 <jats:italic>L. donovani<\/jats:italic> proteins that were identified, the expression of 1387 proteins was detectable in both life stages of the parasite, while 901 and 1423 proteins were identified only in promastigotes and amastigotes life stages, respectively. In addition, we also identified 13 N\u2010terminally and one C\u2010terminally extended proteins based on the proteomic data search against the six\u2010frame translated genome of the three related <jats:italic>Leishmania<\/jats:italic> species. Here, we report results from proteomic profiling of <jats:italic>L. donovani<\/jats:italic>, an organism with an unsequenced genome.<\/jats:p>","DOI":"10.1002\/pmic.201100505","type":"journal-article","created":{"date-parts":[[2012,4,26]],"date-time":"2012-04-26T16:43:52Z","timestamp":1335458632000},"page":"832-844","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["A proteogenomic approach to map the proteome of an unsequenced pathogen \u2013 <i>Leishmania donovani<\/i>"],"prefix":"10.1002","volume":"12","author":[{"given":"Harsh","family":"Pawar","sequence":"first","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bangalore Karnataka India"},{"name":"Rajiv Gandhi University of Health Sciences  Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandini A.","family":"Sahasrabuddhe","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bangalore Karnataka India"},{"name":"Manipal University  Madhav Nagar Manipal Karnataka India"},{"name":"McKusick\u2010Nathans Institute of Genetic Medicine Johns Hopkins University School of Medicine  Baltimore MD USA"},{"name":"Department of Biological Chemistry Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santosh","family":"Renuse","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bangalore Karnataka India"},{"name":"McKusick\u2010Nathans Institute of Genetic Medicine Johns Hopkins University School of Medicine  Baltimore MD USA"},{"name":"Department of Biological Chemistry Johns Hopkins University School of Medicine  Baltimore MD USA"},{"name":"Department of Biotechnology Amrita Vishwa Vidyapeetham  Kollam Kerala India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivakumar","family":"Keerthikumar","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bangalore Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyoti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Institute of Bioinformatics International Technology Park  Bangalore Karnataka India"},{"name":"Manipal University  Madhav Nagar Manipal Karnataka India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ghantasala. 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