{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T01:19:42Z","timestamp":1790817582439,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,9]],"date-time":"2011-12-09T00:00:00Z","timestamp":1323388800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Institute of Development, Aging and Cancer, Tohoku University for generous gift","award":["TS2\/16"],"award-info":[{"award-number":["TS2\/16"]}]},{"name":"JSPS KAKENHI","award":["22659060"],"award-info":[{"award-number":["22659060"]}]},{"name":"Japan Science and Technology Agency and the Naito Foundation"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We previously reported a simple method to analyze the interaction of cell\u2010surface molecules in living cells. This method termed enzyme\u2010mediated activation of radical sources (EMARS) is featured by radical formation of the labeling reagent by horseradish peroxidase (HRP). Herein, we propose an approach to the cell\u2010surface molecular interactome by using combination of this EMARS reaction and MS\u2010based proteomics techniques. In the current study, we employed a novel labeling reagent, fluorescein\u2010conjugated arylazide. The fluorescein\u2010tagged proteins resulting from the EMARS reaction were directly detected in the electrophoresis gels with a fluorescence image analyzer. These products were also purified and concentrated by immunoaffinity chromatography with anti\u2010fluorescein antibody\u2010immobilized resins. The purified fluorescein\u2010tagged proteins were subsequently subjected to an MS\u2010based proteomics analysis. Analysis using HRP\u2010conjugated cholera toxin subunit B, which recognizes a lipid raft marker, ganglioside GM1, revealed 30 membrane and secreted proteins that were candidates for the cell\u2010surface molecules coclustering with GM1. The proposed approach will provide a clue to study functional molecular interactions in a variety of biological events on the cell surface.<\/jats:p>","DOI":"10.1002\/pmic.201100551","type":"journal-article","created":{"date-parts":[[2011,11,22]],"date-time":"2011-11-22T06:17:15Z","timestamp":1321942635000},"page":"54-62","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["A proteomics approach to the cell\u2010surface interactome using the enzyme\u2010mediated activation of radical sources reaction"],"prefix":"10.1002","volume":"12","author":[{"given":"Songlin","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norihiro","family":"Kotani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoko","family":"Ohnishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arisa","family":"Miyagawa\u2010Yamguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayuki","family":"Tsuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryusuke","family":"Yamashita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Ishiura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Honke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,12,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0107-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(99)00047-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.62.070193.002411"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.46.070177.002515"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.5.892"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35004044"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(01)76236-4"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81253-0"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1242\/jcs.101.2.415","article-title":"Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge\u2010coupled device camera. 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