{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:50:28Z","timestamp":1791215428282,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2012,6,28]],"date-time":"2012-06-28T00:00:00Z","timestamp":1340841600000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Jost Reinhold Foundation"},{"name":"Gottfried und Julia Bangerter-Rhyner-Stiftung, Basel"},{"DOI":"10.13039\/501100013850","name":"Helmut Horten Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100013850","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fondazione Ticinese per la Rcicerca sul Cancro"},{"name":"ANR","award":["ANR-10-JCJC 1104 01"],"award-info":[{"award-number":["ANR-10-JCJC 1104 01"]}]},{"DOI":"10.13039\/501100007486","name":"Universit\u00e9 Paris-Sud","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100007486","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004099","name":"Ligue Nationale Contre le Cancer","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004099","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ANR"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2012,6]]},"abstract":"<jats:p>PTMs of extracellular domains of membrane proteins can influence antibody binding and give rise to ambivalent results. Best proof of protein expression is the use of complementary methods to provide unequivocal evidence. <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7, a member of the atypical chemokine receptor family, mainly functions as scavenger for the chemokines <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>12 and <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>11. The expression of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7 on nonhematopoietic cells and neoplasms is widely accepted, however, its expression on leukocytes was recently challenged. To solve the dissent, we thoroughly analyzed the expression of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7 on human <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. We validated the efficiency of different epitope\u2010specific monoclonal antibodies to detect <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7 on transfected cells and primary human <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. The specificity of the used antibodies was further confirmed by an experimentally independent double labeling approach. Examination of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7\u2010dependent scavenging of fluorescent\u2010labeled <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>12 revealed functional expression of the receptor on human <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. Moreover, real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> showed the presence of transcripts in human leukocytes. Finally, two <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7\u2010specific peptides were identified by MS in immunoprecipitates from primary human <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells. Thus, we present a strategy based on combined proteomic and functional approaches that can be used to solve dissents on protein expression, i.e. demonstrating the expression of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>7 on human leukocytes.<\/jats:p>","DOI":"10.1002\/pmic.201100581","type":"journal-article","created":{"date-parts":[[2012,5,23]],"date-time":"2012-05-23T09:44:28Z","timestamp":1337766268000},"page":"1938-1948","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Complementary methods provide evidence for the expression of <scp>CXCR<\/scp>7 on human <scp>B<\/scp> cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Marie\u2010Luise","family":"Humpert","sequence":"first","affiliation":[{"name":"Insitute for Research in Biomedicine Bellinzona Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Tzouros","sequence":"additional","affiliation":[{"name":"F. Hoffmann\u2010La Roche Ltd, TRS \u2010 CCC Proteins and Metabolites, Functional and Pathway Proteomics Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Thelen","sequence":"additional","affiliation":[{"name":"Insitute for Research in Biomedicine Bellinzona Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre","family":"Bignon","sequence":"additional","affiliation":[{"name":"Laboratoire Cytokines, Chemokines and Immunopathology, Univ. Paris\u2010Sud Clamart France"},{"name":"INSERM, Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) Clamart France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang\u00e9lique","family":"Levoye","sequence":"additional","affiliation":[{"name":"Department of Virology, INSERM U819 &amp; Laboratory of Viral Pathogenesis, Institut Pasteur Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"Arenzana\u2010Seisdedos","sequence":"additional","affiliation":[{"name":"Department of Virology, INSERM U819 &amp; Laboratory of Viral Pathogenesis, Institut Pasteur Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl","family":"Balabanian","sequence":"additional","affiliation":[{"name":"Laboratoire Cytokines, Chemokines and Immunopathology, Univ. Paris\u2010Sud Clamart France"},{"name":"INSERM, Laboratory of Excellence in Research on Medication and Innovative Therapeutics (LERMIT) Clamart France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Bachelerie","sequence":"additional","affiliation":[{"name":"Department of Virology, INSERM U819 &amp; Laboratory of Viral Pathogenesis, Institut Pasteur Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanno","family":"Langen","sequence":"additional","affiliation":[{"name":"F. Hoffmann\u2010La Roche Ltd, TRS \u2010 CCC Proteins and Metabolites, Functional and Pathway Proteomics Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Thelen","sequence":"additional","affiliation":[{"name":"Insitute for Research in Biomedicine Bellinzona Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,6,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.46.120604.141218"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm908"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/84224"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1252"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.4.504"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80577-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.19.8957-8967.2001"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2011.03485.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-196618"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1964"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2011.01.012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.12.034"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.212"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0009175"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1182"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535716"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH07-02-0105"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052144"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900269"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20102010"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912852107"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.062810"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.09724"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.108.053389"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.277038"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702229104"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.08-116749"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.12.031"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.12.006"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2010.12.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508234200"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-10-73"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-010-9256-x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001660"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.4.2197"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24612"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1565"},{"key":"e_1_2_7_39_1","article-title":"B cell homeostasis and plasma cell homing controlled by Kruppel\u2010like factor 2","author":"Winkelmann R.","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0208088"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.2.619-626.2000"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.3.1941"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-12-258186"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0111101"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-03-343608"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.11.006"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.09.050"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200111)31:11<3388::AID-IMMU3388>3.0.CO;2-Q"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T500030-MCP200"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1021\/cb700054k"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2298-2305.2006"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja048396s"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.1.139"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm801065q"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201004052"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M106229200"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-07-102103"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.2144\/000113126"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20071903"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-6997(24)01439-X"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.657"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0914248107"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e328338b7d5"},{"key":"e_1_2_7_64_1","article-title":"AMD3100 is a potent antagonist at CXCR4 (R334X), a hyperfunctional mutant chemokine receptor and cause of WHIM syndrome","author":"McDermott D. H.","year":"2010","journal-title":"J. Cell Mol. Med."},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1844"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0610444104"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.34.23916"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838906"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2006.04.040"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201100581","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201100581","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201100581","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:00:34Z","timestamp":1761048034000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201100581"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,6]]},"references-count":68,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2012,6]]}},"alternative-id":["10.1002\/pmic.201100581"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201100581","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,6]]},"assertion":[{"value":"2011-11-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-03-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}