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It is desirable to assess hepatic fibrosis with noninvasive means. Targeted proteomic techniques allow an unbiased assessment of proteins and might be useful to identify proteins related to hepatic fibrosis. We utilized selected reaction monitoring (<jats:styled-content style=\"fixed-case\">SRM<\/jats:styled-content>) targeted proteomics combined with an organ\u2010specific blood protein strategy to identify and quantify 38 liver\u2010specific proteins. A combination of protein <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> and retinol\u2010binding protein 4 in serum gave promising preliminary results as candidate biomarkers to distinguish patients at different stages of hepatic fibrosis due to chronic infection with hepatitis <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HCV<\/jats:styled-content>). Also, alpha\u20101\u2010<jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> glycoprotein, complement factor <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> and insulin\u2010like growth factor binding protein acid labile subunit performed well in distinguishing patients from healthy controls.<\/jats:p>","DOI":"10.1002\/pmic.201100601","type":"journal-article","created":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T03:57:50Z","timestamp":1336708670000},"page":"1244-1252","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["<scp>SRM<\/scp> targeted proteomics in search for biomarkers of <scp>HCV<\/scp>\u2010induced progression of fibrosis to cirrhosis in <scp>HALT<\/scp>\u2010<scp>C<\/scp> patients"],"prefix":"10.1002","volume":"12","author":[{"given":"Shizhen","family":"Qin","sequence":"first","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna S.","family":"Lok","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Tsodikov","sequence":"additional","affiliation":[{"name":"Department of Biostatistics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowei","family":"Yan","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Gray","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yuan","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Moritz","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Galas","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilbert S.","family":"Omenn","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"},{"name":"Department of Internal Medicine and Center for Computational Medicine and Bioinformatics University of Michigan  Ann Arbor Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leroy","family":"Hood","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology  Seattle Washington USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22759"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2002.06038.x"},{"key":"e_1_2_8_4_1","first-page":"187","article-title":"Noninvasive biomarkers for the screening of fibrosis, steatosis and steatohepatitis in patients with metabolic risk factors: FibroTest\u2010FibroMax experience","volume":"17","author":"Munteanu M.","year":"2008","journal-title":"J. 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