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Here, we investigated early changes in the liver\u2010soluble proteome of female <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content> mice fed an <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>\u2010inducing diet by 2D\u2010DIGE and nano\u2010<jats:styled-content style=\"fixed-case\">HPLC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>. In parallel, histology and measurements of hepatic content of triglycerides, cholesterol and intermediates of the methionine cycle were performed. Hepatic steatosis manifested itself after 2 days of feeding, albeit significant changes in the liver\u2010soluble proteome were not evident before day 10 in the absence of inflammatory or fibrotic signs. Proteomic alterations affected mainly energy and amino acid metabolism, detoxification processes, urea cycle, and the one\u2010carbon\/<jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>\u2010adenosylmethionine pathways. Additionally, intermediates of relevant affected pathways were quantified from liver tissue, confirming the findings from the proteomic analysis.<\/jats:p>","DOI":"10.1002\/pmic.201100628","type":"journal-article","created":{"date-parts":[[2012,5,16]],"date-time":"2012-05-16T06:41:08Z","timestamp":1337150468000},"page":"1437-1451","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Early changes in the liver\u2010soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet"],"prefix":"10.1002","volume":"12","author":[{"given":"Anja","family":"Thomas","sequence":"first","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel P.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias S.","family":"Klein","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"Hellerbrand","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine I University Hospital Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Dettmer","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram","family":"Gronwald","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Oefner","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Reinders","sequence":"additional","affiliation":[{"name":"Institute of Functional Genomics University of Regensburg Regensburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1159\/000282080"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i18.2223"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70599-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1262-3636(07)70098-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2004.09.021"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20023"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00521.2005"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2002.32527"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0959-9673.2006.00465.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M306022200"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2010.03.006"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20838"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2007.02.003"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365520903353379"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20080159"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0536625100"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0607390"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7601934"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00490123"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"e_1_2_7_22_1","first-page":"505","article-title":"Expression of fatty acid synthase in nonalcoholic fatty liver disease","volume":"25","author":"Dorn C.","year":"2010","journal-title":"Int. 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