{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T10:10:57Z","timestamp":1792145457401,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T00:00:00Z","timestamp":1336694400000},"content-version":"vor","delay-in-days":40,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Health grant","award":["1S10RR027643-01"],"award-info":[{"award-number":["1S10RR027643-01"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:p>\n                    The epigenetic remodeling of chromatin histone proteins by acetylation has been the subject of recent investigations searching for biomarkers indicative of late onset cognitive loss. Histone acetylations affect the regulation of gene transcription, and the loss of learning induced deacetylation at specific histone sites may represent biomarkers for memory loss and\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    lzheimer's disease (\n                    <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>\n                    ). Selected\u2010reaction\u2010monitoring (\n                    <jats:styled-content style=\"fixed-case\">SRM<\/jats:styled-content>\n                    ) has recently been advanced to quantitate peptides and proteins in complex biological systems. In this paper, we provide evidence that\n                    <jats:styled-content style=\"fixed-case\">SRM<\/jats:styled-content>\n                    \u2010based targeted proteomics can reliably quantify specific histone acetylations in both AD and control brain by identifying the patterns of\n                    <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                    3\n                    <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>\n                    18\/K23 acetylations Results of targeted proteomics assays have been validated by\n                    <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>\n                    estern blot (\n                    <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>\n                    ) analysis. As compared with\n                    <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    \/\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">TMT<\/jats:styled-content>\n                    (tandem\u2010mass\u2010tagging) and\n                    <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>\n                    methods, the targeted proteomics method has shown higher throughput, and therefore promised to be more suitable for clinical applications. With this methodology, we find that histone acetylation is significantly lower in\n                    <jats:styled-content style=\"fixed-case\">AD<\/jats:styled-content>\n                    temporal lobe than found in aged controls. Targeted proteomics warrants increased application for studying epigenetics of neurodegenerative diseases.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201200010","type":"journal-article","created":{"date-parts":[[2012,5,11]],"date-time":"2012-05-11T03:57:50Z","timestamp":1336708670000},"page":"1261-1268","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":180,"title":["Targeted proteomics for quantification of histone acetylation in\n                    <scp>A<\/scp>\n                    lzheimer's disease"],"prefix":"10.1002","volume":"12","author":[{"given":"Kangling","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Basic Sciences School of Medicine Loma Linda University  Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Schrag","sequence":"additional","affiliation":[{"name":"Department of Neurology Yale University  New Haven CT USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Crofton","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences School of Medicine Loma Linda University  Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rishi","family":"Trivedi","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences School of Medicine Loma Linda University  Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Vinters","sequence":"additional","affiliation":[{"name":"Section of Neuropathology Department of Neurology University of California  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolff","family":"Kirsch","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences School of Medicine Loma Linda University  Loma Linda CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700354-MCP200"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.138420"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1586\/epr.09.11"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-009-2752-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/eln056"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.329"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1504"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900375-MCP200"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac901278y"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.204800"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2009-1134"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2011-110080"},{"key":"e_1_2_7_14_1","unstructured":"Ricobaraza A. Cuadrado\u2010Tejedor M. Marco S. P\u00e9rez\u2010Ota\u00f1o I.et al. Phenylbutyrate rescues dentritic spine loss associated with memory deficits in a mouse model ofAlzheimer disease.Hippocampus(in press)."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2010.02.055"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2697(03)00032-0"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2006.08.017"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900777e"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.126813"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05772"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1186088"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/npp.2009.197"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.10845"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2009.131"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986711795496872"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200010","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200010","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201200010","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:10:52Z","timestamp":1761048652000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201200010"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,4]]},"references-count":24,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2012,4]]}},"alternative-id":["10.1002\/pmic.201200010"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201200010","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,4]]},"assertion":[{"value":"2011-08-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-01-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-05-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}