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By comparing proteomes of the cerebrospinal fluid (CSF<\/jats:styled-content>) before and after the treatment, it may be possible to identify proteins that play a role in PHN<\/jats:styled-content> and to predict responses to various treatments. To address this issue, we enrolled eight outpatients with PHN<\/jats:styled-content> over 55 years of age and treated them with intrathecal methylprednisolone and lidocaine four times every week, collecting CSF<\/jats:styled-content> samples before the treatment at each visit. We used 2D DIGE<\/jats:styled-content> to investigate differentially expressed proteins in the CSF<\/jats:styled-content> before and after repetitive treatments individually. Of 145 differentially expressed spots, the levels of nine proteins were decreased by the treatment including lipocalin\u2010type prostaglandin D<\/jats:styled-content> synthase (L<\/jats:styled-content>\u2010PGDS<\/jats:styled-content>), and five were increased by it. The time course of alterations in the L<\/jats:styled-content>\u2010PGDS<\/jats:styled-content> concentration in the CSF<\/jats:styled-content> of each patient, detected by a pairwise and sandwich ELISA<\/jats:styled-content> by SPR<\/jats:styled-content> constructed here was well correlated with that by 1DE W<\/jats:styled-content>estern blots with anti\u2010L<\/jats:styled-content>\u2010PGDS<\/jats:styled-content> antibody, but was not related with that of the pain relief. The present study demonstrates that the real\u2010time ELISA<\/jats:styled-content> was precise and sensitive enough to measure L<\/jats:styled-content>\u2010PGDS<\/jats:styled-content> in the CSF<\/jats:styled-content> and that the steroid treatment decreased the L<\/jats:styled-content>\u2010PGDS<\/jats:styled-content> concentration in CSF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/pmic.201200125","type":"journal-article","created":{"date-parts":[[2012,8,31]],"date-time":"2012-08-31T06:07:41Z","timestamp":1346393261000},"page":"3105-3112","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteomic analysis of cerebrospinal fluid before and after intrathecal injection of steroid into patients with postherpetic pain"],"prefix":"10.1002","volume":"12","author":[{"given":"Jingshan","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Medical Chemistry Kansai Medical University Moriguchi 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