{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:23:13Z","timestamp":1790673793658,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2013,8,9]],"date-time":"2013-08-09T00:00:00Z","timestamp":1376006400000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["EY017731"],"award-info":[{"award-number":["EY017731"]}]},{"name":"NIH","award":["EY012689"],"award-info":[{"award-number":["EY012689"]}]},{"name":"NIH","award":["EY03040"],"award-info":[{"award-number":["EY03040"]}]},{"DOI":"10.13039\/501100001349","name":"National Medical Research Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001349","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,8]]},"abstract":"\n Sj\u00f6gren's syndrome (\n SS<\/jats:styled-content>\n ) is an autoimmune disease that results in pathological dryness of mouth and eye. The diagnosis of\n SS<\/jats:styled-content>\n depends on both clinical evaluation and specific antibodies. The goal of this study was to use quantitative proteomics to investigate changes in tear proteins in a rabbit model of\n SS<\/jats:styled-content>\n \u2010associated dry eye, induced autoimmune dacryoadenitis (\n IAD<\/jats:styled-content>\n ). Proteomic analysis was performed by i\n TRAQ<\/jats:styled-content>\n and nano\n LC<\/jats:styled-content>\n \u2010\n MS<\/jats:styled-content>\n \/\n MS<\/jats:styled-content>\n on tears collected from the ocular surface, and specific proteins were verified by high resolution\n MRM<\/jats:styled-content>\n . It was found that in the tears of\n IAD<\/jats:styled-content>\n rabbits at 2 and 4 weeks after induction,\n S<\/jats:styled-content>\n 100\n A<\/jats:styled-content>\n 6, S\n 1<\/jats:styled-content>\n 00\n A<\/jats:styled-content>\n 9, and serum albumin were upregulated, whereas serotransferrin (\n TF<\/jats:styled-content>\n ), prolactin\u2010inducible protein (\n PIP<\/jats:styled-content>\n ), polymeric immunoglobulin receptor (p\n I<\/jats:styled-content>\n g\n R<\/jats:styled-content>\n ), and\n I<\/jats:styled-content>\n g gamma chain\n C<\/jats:styled-content>\n region were downregulated. High resolution\n MRM<\/jats:styled-content>\n with m\n TRAQ<\/jats:styled-content>\n labeling verified the changes in\n S<\/jats:styled-content>\n 100\n A<\/jats:styled-content>\n 6,\n TF<\/jats:styled-content>\n ,\n PIP<\/jats:styled-content>\n , and p\n I<\/jats:styled-content>\n g\n R<\/jats:styled-content>\n . Our results indicated significant changes of tear proteins in\n IAD<\/jats:styled-content>\n rabbits, suggesting these proteins could potentially be used as biomarkers for the diagnosis and prognosis of dry eye. Several of these proteins were also found in the tears of non\u2010\n SS<\/jats:styled-content>\n dry eye patients indicating a common basis of ocular surface pathology, however, p\n I<\/jats:styled-content>\n g\n R<\/jats:styled-content>\n appears to be unique to\n SS<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1002\/pmic.201200230","type":"journal-article","created":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T09:44:11Z","timestamp":1370252651000},"page":"2469-2481","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["Proteomic analysis revealed the altered tear protein profile in a rabbit model of\n S<\/scp>\n j\u00f6gren's syndrome\u2010associated dry eye"],"prefix":"10.1002","volume":"13","author":[{"given":"Lei","family":"Zhou","sequence":"first","affiliation":[{"name":"Singapore Eye Research Institute Singapore"},{"name":"Department of Ophthalmology Yong Loo Lin School of Medicine National University of Singapore"},{"name":"SRP Neuroscience and Behavioral Disorder DUKE\u2010NUS Graduate Medical School Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruihua","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Cell and Neurobiology University of Southern California Keck School of Medicine Los Angeles CA USA"},{"name":"Doheny Eye Institute Los Angeles CA USA"},{"name":"Tianjin Medical University Eye Center Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Cell and Neurobiology University of Southern California Keck School of Medicine Los Angeles CA USA"},{"name":"Doheny Eye Institute Los Angeles CA USA"},{"name":"Department of Ophthalmology Third Hospital of Hebei Medical University Shijiazhuang Hebei, P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siew Kwan","family":"Koh","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger W.","family":"Beuerman","sequence":"additional","affiliation":[{"name":"Singapore Eye Research Institute Singapore"},{"name":"Department of Ophthalmology Yong Loo Lin School of Medicine National University of Singapore"},{"name":"SRP Neuroscience and Behavioral Disorder DUKE\u2010NUS Graduate Medical School Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuanqing","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Cell and Neurobiology University of Southern California Keck School of Medicine Los Angeles CA USA"},{"name":"Doheny Eye Institute Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199801000-00007"},{"key":"e_1_2_7_3_1","first-page":"1666","article-title":"Mikulicz's disease and Sjogren's syndrome","volume":"41","author":"Tsubota K.","year":"2000","journal-title":"Invest. 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