{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T16:10:09Z","timestamp":1761063009959,"version":"build-2065373602"},"reference-count":10,"publisher":"Wiley","issue":"10-11","license":[{"start":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T00:00:00Z","timestamp":1370217600000},"content-version":"vor","delay-in-days":33,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p>Antibody arrays have been used as an effective method for simultaneously detecting multiple proteins such as cytokines. However, their use in quantifying a large number of cellular proteins has the following limitations: (i) unsuitable for simultaneously detecting proteins that may form complexes with each other; (ii) incapable of quantitatively detecting more than one epitope of each protein such as phospho\u2010 and nonphospho\u2010epitopes; and (iii) incapable of simultaneously detecting multiple biomarkers on solid surfaces such as formalin\u2010fixed tissue sections. Using a novel multiple epitope detection (MED) technique, we have overcome these limitations and have improved upon currently available antibody\u2010based protein detection technologies. The MED technique employs primary antibody detection of epitopes within fixed cells or tissue, followed by elution of bound antibodies, and subsequent quantification of the eluted antibodies by an epitope array. Using the MED method, we demonstrate accurate detection of individual proteins even in complex with each other, simultaneous quantitative detection of phospho\u2010 and nonphospho\u2010epitopes on a protein, and sensitive detection of multiple biomarkers on formalin\u2010fixed tissue sections.<\/jats:p>","DOI":"10.1002\/pmic.201200381","type":"journal-article","created":{"date-parts":[[2013,4,2]],"date-time":"2013-04-02T04:04:51Z","timestamp":1364875491000},"page":"1696-1700","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Multiplex epitope detection: A new method overcomes limitations of antibody arrays"],"prefix":"10.1002","volume":"13","author":[{"given":"Hui","family":"Cen","sequence":"first","affiliation":[{"name":"LEAP Biosciences Corporation  Palo Alto CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Windler","sequence":"additional","affiliation":[{"name":"LEAP Biosciences Corporation  Palo Alto CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lyle S.","family":"Rice","sequence":"additional","affiliation":[{"name":"LEAP Biosciences Corporation  Palo Alto CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy","family":"Zhang","sequence":"additional","affiliation":[{"name":"Epitomics, Inc  Burlingame CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Zhou","sequence":"additional","affiliation":[{"name":"Epitomics, Inc  Burlingame CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,3]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-043-0_1"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1999.00028.x"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030447"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1037"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600324"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfq100"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.T500032-MCP200"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2009.10.016"},{"key":"e_1_2_4_10_1","first-page":"169","article-title":"Immunohistochemistry in diagnostic surgical pathology: contributions of protein lifecycle, use of evidence\u2010based methods and data normalization on interpretation of immunohistochemical stains","volume":"3","author":"Idikio H. 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