{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T01:50:28Z","timestamp":1788313828204,"version":"build-2803163510"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,1,13]],"date-time":"2014-01-13T00:00:00Z","timestamp":1389571200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003653","name":"Korea National Institute of Health","doi-asserted-by":"crossref","award":["4840-300-210-13"],"award-info":[{"award-number":["4840-300-210-13"]}],"id":[{"id":"10.13039\/501100003653","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p>\n                    Anthrax is caused by the spore\u2010forming bacterium\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      acillus anthracis\n                    <\/jats:italic>\n                    , which has been used as a weapon for bioterrorism. Although current vaccines are effective, they involve prolonged dose regimens and often cause adverse reactions. High rates of mortality associated with anthrax have made the development of an improved vaccine a top priority. To identify novel vaccine candidates, we applied an immunoproteomics approach. Using sera from convalescent guinea pigs or from human patients with anthrax, we identified 34 immunogenic proteins from the virulent\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      . anthracis\n                    <\/jats:italic>\n                    <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\n                    9401. To evaluate vaccine candidates, six were expressed as recombinant proteins and tested in vivo. Two proteins, r\n                    <jats:styled-content style=\"fixed-case\">GBAA<\/jats:styled-content>\n                    _0345 (alkyl hydroperoxide reductase subunit\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    ) and r\n                    <jats:styled-content style=\"fixed-case\">GBAA<\/jats:styled-content>\n                    _3990 (malonyl\n                    <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>\n                    o\n                    <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\n                    \u2010acyl carrier protein transacylase), have afforded guinea pigs partial protection from a subsequent virulent\u2010spore challenge. Moreover, combined vaccination with r\n                    <jats:styled-content style=\"fixed-case\">GBAA<\/jats:styled-content>\n                    _0345 and r\n                    <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\n                    (protective antigen) exhibited an enhanced ability to protect against anthrax mortality. Finally, we demonstrated that\n                    <jats:styled-content style=\"fixed-case\">GBAA<\/jats:styled-content>\n                    _0345 localizes to anthrax spores and bacilli. Our results indicate that r\n                    <jats:styled-content style=\"fixed-case\">GBAA<\/jats:styled-content>\n                    _0345 may be a potential component of a multivalent anthrax vaccine, as it enhances the efficacy of r\n                    <jats:styled-content style=\"fixed-case\">PA<\/jats:styled-content>\n                    vaccination. This is the first time that sera from patients with anthrax have been used to interrogate the proteome of virulent\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\n                      . anthracis\n                    <\/jats:italic>\n                    vegetative cells.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201200495","type":"journal-article","created":{"date-parts":[[2013,11,25]],"date-time":"2013-11-25T06:27:12Z","timestamp":1385360832000},"page":"93-104","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Immunoproteomically identified\n                    <scp>GBAA<\/scp>\n                    _0345, alkyl hydroperoxide reductase subunit\n                    <scp>C<\/scp>\n                    is a potential target for multivalent anthrax vaccine"],"prefix":"10.1002","volume":"14","author":[{"given":"Yeon Hee","family":"Kim","sequence":"first","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"},{"name":"School of Life Sciences and Biotechnology Korea University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyung Ae","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Ri","family":"Kim","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Kyung","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Kyeong","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Bioscience &amp; Biotechnology Institute of Bioscience, Sejong University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ki Ju","family":"Choi","sequence":"additional","affiliation":[{"name":"Division of Respiratory Viruses Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeong\u2010Hoon","family":"Chun","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiweon","family":"Cha","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kee\u2010Jong","family":"Hong","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na Gyong","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Bioscience &amp; Biotechnology Institute of Bioscience, Sejong University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheon\u2010Kwon","family":"Yoo","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee\u2010Bok","family":"Oh","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tae Sung","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology Korea University Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gi\u2010eun","family":"Rhie","sequence":"additional","affiliation":[{"name":"Division of High\u2010risk Pathogen Research Korea National Institute of Health Chungbuk Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.55.1.647"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.07.004"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.17.2236"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909093411107"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052160199"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid0810.020380"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.04.036"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.102"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.7.2.219"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00152686"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0264-410X(91)90237-Z"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2009.08.006"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.05.044"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.3.5.4459"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsim.2005.05.008"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.07.004"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(98)80112-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5978-5987.2005"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.12.028"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)00268-5"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12272-001-2121-2"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.4161\/hv.3.3.4011"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1086\/422694"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1834478100"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.52.2.509-512.1986"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.2.661-664.2002"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300844"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900439j"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr1000219"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.09.017"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2010.00969.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00455-06"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5685-5696.2005"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.6.1903-1910.2003"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4563-4579.2003"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300575"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00174-06"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02029-06"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-5-11"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.10.3551-3571.2006"},{"key":"e_1_2_8_42_1","article-title":"Two Cases of Gastrointestinal Anthrax","volume":"29","author":"Kim C. 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