{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:20:39Z","timestamp":1780568439082,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2013,7,30]],"date-time":"2013-07-30T00:00:00Z","timestamp":1375142400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"German Statutory Accident Insurance"},{"name":"Australian Governments NCRIS\/EIF programs"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:p>In contrast to normal healing wounds, chronic wounds commonly show disturbances in proteins regulating wound healing processes, particularly those involved in cell proliferation and protein degradation. Multidimensional protein identification technology MS\/MS was conducted to investigate and compare the protein composition of chronic diabetic foot exudates to exudates from split\u2010skin donor sites of burn victims otherwise healthy. Spectral counting revealed 188 proteins differentially expressed (more than twofold and <jats:italic>p<\/jats:italic>\u2010value &lt;0.05) in chronic wounds. Most were involved in biological processes including inflammation, angiogenesis, and cell mortality. Increased expression of the inflammatory response stimulating S100 proteins, predominantly S100A8 and S100A9 (almost tenfold), was identified. Matrix metalloproteinases (MMPs) MMP1, MMP2, and MMP8 were identified to be elevated in chronic wounds with significant impact on collagen degradation and tissue destruction. Further, proteins with antiangiogenic properties were found at higher expression levels in chronic wounds. Reduced angiogenesis leads to drastic shortage in nutrition supply and causes increased cell death, demonstrated by Annexin A5 exclusively found in chronic wound exudates. However, excessive nucleic and cytosolic material infers cell death occurring not only by apoptosis but also by necrosis. In conclusion, mass spectrometric investigation of exudates from chronic wounds demonstrated dramatic impairment in wound repair with excessive inflammation, antiangiogenic environment, and accelerated cell death.<\/jats:p>","DOI":"10.1002\/pmic.201200502","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T06:46:17Z","timestamp":1372142777000},"page":"2670-2681","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":111,"title":["Proteome analysis reveals antiangiogenic environments in chronic wounds of diabetes mellitus type 2 patients"],"prefix":"10.1002","volume":"13","author":[{"given":"Christoph","family":"Krisp","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Biomolecular Mass Spectrometry Ruhr\u2010University Bochum  Bochum Germany"},{"name":"Department of Chemistry and Biomolecular Sciences Macquarie University  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Jacobsen","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Oncology and Wound Repair Department of Plastic Surgery Ruhr\u2010University Bochum  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"McKay","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Sciences Macquarie University  Sydney Australia"},{"name":"Australian Proteome Analysis Facility (APAF) Macquarie University  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Molloy","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biomolecular Sciences Macquarie University  Sydney Australia"},{"name":"Australian Proteome Analysis Facility (APAF) Macquarie University  Sydney Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Steinstraesser","sequence":"additional","affiliation":[{"name":"Laboratory for Molecular Oncology and Wound Repair Department of Plastic Surgery Ruhr\u2010University Bochum  Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk A.","family":"Wolters","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Biomolecular Mass Spectrometry Ruhr\u2010University Bochum  Bochum Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.122.2.417"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/153857440503900401"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2002.3.s1-23"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2011.01309.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2337\/dc08-0763"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4049"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2008.02414.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1999.00738.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clindermatol.2004.07.020"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000155223"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(98)00183-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-5491.2006.01773.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2004.06175.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr100456d"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2007.12.043"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318171971a"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.324.7330.160"},{"key":"e_1_2_7_19_1","first-page":"119","article-title":"Composition of wound fluid from pressure ulcers treated with negative pressure wound therapy using VAC (R) therapy in home health or extended care patients: a pilot study","volume":"18","author":"Kilpadi D. V.","year":"2006","journal-title":"Wounds"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/jsre.2000.5892"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080765"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.2002.10502.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(91)90040-L"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/85686"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac010617e"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900373"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4204"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.6.1615"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2796.2002.00932.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2005.01534.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00547.x"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jvs.2011.05.029"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.17.12799"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1998.00457.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1155\/S1110724304307096"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb1661iti2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1993.1298"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(199601)178:1<30::AID-PATH442>3.0.CO;2-7"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2008.08904.x"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.71"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2708"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2053"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.139.4.510"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200502","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201200502","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201200502","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:49:22Z","timestamp":1761058162000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201200502"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,30]]},"references-count":42,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1002\/pmic.201200502"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201200502","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,30]]},"assertion":[{"value":"2012-11-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-07-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}