{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:59:43Z","timestamp":1788872383965,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T00:00:00Z","timestamp":1372118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Science for Life Laboratory Stockholm, ProNova VINN Excellence Centre for Protein Technology"},{"DOI":"10.13039\/501100004063","name":"Knut and Alice Wallenberg Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:p>\n                    Antibody suspension bead arrays have proven to enable multiplexed and high\u2010throughput protein profiling in unfractionated plasma and serum samples through a direct labeling approach. We here describe the development and application of an assay for protein profiling of cerebrospinal fluid (\n                    <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\n                    ). While setting up the assay, systematic intensity differences between sample groups were observed that reflected inherent sample specific total protein amounts. Supplementing the labeling reaction with\n                    <jats:styled-content style=\"fixed-case\">BSA<\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>\n                    g\n                    <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\n                    diminished these differences without impairing the apparent sensitivity of the assay. We also assessed the effects of heat treatment on the analysis of\n                    <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\n                    proteins and applied the assay to profile 43 selected proteins by 101 antibodies in 339\n                    <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\n                    samples from a multiple sclerosis (\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    ) cohort. Two proteins,\n                    <jats:styled-content style=\"fixed-case\">GAP<\/jats:styled-content>\n                    43 and SERPINA3 were found to have a discriminating potential with altered intensity levels between sample groups.\n                    <jats:styled-content style=\"fixed-case\">GAP<\/jats:styled-content>\n                    43 was detected at significantly lower levels in secondary progressive\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    compared to early stages of\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    and the control group of other neurological diseases. SERPINA3 instead was detected at higher levels in all\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    patients compared to controls. The developed assay procedure now offers new possibilities for broad\u2010scale protein profiling of\n                    <jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>\n                    within neurological disorders.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201200580","type":"journal-article","created":{"date-parts":[[2013,5,21]],"date-time":"2013-05-21T05:22:49Z","timestamp":1369113769000},"page":"2256-2267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Antibody\u2010based profiling of cerebrospinal fluid within multiple sclerosis"],"prefix":"10.1002","volume":"13","author":[{"given":"Anna","family":"H\u00e4ggmark","sequence":"first","affiliation":[{"name":"SciLifeLab Stockholm School of Biotechnology KTH Royal Institute of Technology  Stockholm 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