{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T07:20:39Z","timestamp":1777533639130,"version":"3.51.4"},"reference-count":13,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2013,10,8]],"date-time":"2013-10-08T00:00:00Z","timestamp":1381190400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p>We describe the development of a novel, robust assay system for determining the changes in activity of proprotein converting enzymes. An assay for prolyl oligopeptidase (<jats:styled-content style=\"fixed-case\">POP<\/jats:styled-content>) activity was constructed using a peptide\u2010streptavidin substrate coupled to magnetic microspheres and cleavage was detected by loss of streptavidin on the <jats:styled-content style=\"fixed-case\">MAGPIX<\/jats:styled-content> reader. Test analysis of postmortem pituitary extracts from schizophrenia patients showed an increase in <jats:styled-content style=\"fixed-case\">POP<\/jats:styled-content> activity compared to controls. The results were validated using both fluorometric and <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blot analyses for <jats:styled-content style=\"fixed-case\">POP<\/jats:styled-content> activity and immunoreactivity, respectively. The assays can be multiplexed for measuring the activity of multiple proprotein cleaving enzymes simultaneously in laboratory and clinical settings and should add valuable new information for conditions such as neuropsychiatric diseases, diabetes, endocrine dysfunction, and cancer, where effects on proteolysis of biologically active peptides play a key role.<\/jats:p>","DOI":"10.1002\/pmic.201300060","type":"journal-article","created":{"date-parts":[[2013,8,23]],"date-time":"2013-08-23T03:26:49Z","timestamp":1377228409000},"page":"2976-2979","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of a novel assay for proprotein converting enzyme activity on a multiplex bead\u2010based array system"],"prefix":"10.1002","volume":"13","author":[{"given":"Andreas","family":"Albrecht","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hassan","family":"Rahmoune","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristi","family":"Leedj\u00e4rv","sequence":"additional","affiliation":[{"name":"Storkbio Ltd Tallinn Estonia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Knorpp","sequence":"additional","affiliation":[{"name":"EDI GmbH Reutlingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Joos","sequence":"additional","affiliation":[{"name":"EDI GmbH Reutlingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawel","family":"Stocki","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul C.","family":"Guest","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"Bahn","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Biotechnology University of Cambridge Cambridge UK"},{"name":"Department of Neuroscience Erasmus MC Rotterdam The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,8]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00238-6"},{"key":"e_1_2_4_3_1","first-page":"633","article-title":"Key factors in evaluating potential clinical biomarkers","volume":"10","author":"Spain M.","year":"2007","journal-title":"Drugs"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-204-5_17"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M803762200"},{"key":"e_1_2_4_6_1","first-page":"235","article-title":"A sensitive method for the assay of serum prolyl endopeptidase","volume":"30","author":"Goossens F.","year":"1992","journal-title":"Eur. 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