{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T03:48:19Z","timestamp":1789444099744,"version":"build-2803163510"},"reference-count":70,"publisher":"Wiley","issue":"23-24","license":[{"start":{"date-parts":[[2013,12,16]],"date-time":"2013-12-16T00:00:00Z","timestamp":1387152000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Army Research Office of the Department of Defense"},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["RO1 AI071752"],"award-info":[{"award-number":["RO1 AI071752"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["R21 AI083718"],"award-info":[{"award-number":["R21 AI083718"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:p>\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    , the main causative agent of cryptococcosis, is a fungal pathogen that causes life\u2010threatening meningoencephalitis in immunocompromised patients. To date, there is no vaccine or immunotherapy approved to treat cryptococcosis. Cell\u2010 and antibody\u2010mediated immune responses collaborate to mediate optimal protection against\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    infections. Accordingly, we identified cryptococcal protein fractions capable of stimulating cell\u2010 and antibody\u2010mediated immune responses and determined their efficacy to elicit protection against cryptococcosis. Proteins were extracted from\n                    <jats:italic>C. neoformans<\/jats:italic>\n                    and fractionated based on molecular mass. The fractions were then evaluated by immunoblot analysis for reactivity to serum extracted from protectively immunized mice and in cytokine recall assays for their efficacy to induce pro\u2010inflammatory and\n                    <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\n                    h1\u2010type cytokine responses associated with protection.\n                    <jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\n                    analysis revealed a number of proteins with roles in stress response, signal transduction, carbohydrate metabolism, amino acid synthesis, and protein synthesis. Immunization with select protein fractions containing immunodominant antigens induced significantly prolonged survival against experimental pulmonary cryptococcosis. Our studies support using the combination of immunological and proteomic approaches to identify proteins that elicit antigen\u2010specific antibody and\n                    <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\n                    h1\u2010type cytokine responses. The immunodominant antigens that were discovered represent attractive candidates for the development of novel subunit vaccines for treatment and\/or prevention of cryptococcosis.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201300213","type":"journal-article","created":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T20:39:06Z","timestamp":1383079146000},"page":"3429-3441","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Identification and characterization of\n                    <i>\n                      <scp>C<\/scp>\n                      ryptococcus neoformans\n                    <\/i>\n                    protein fractions that induce protective immune responses"],"prefix":"10.1002","volume":"13","author":[{"given":"Ashok K.","family":"Chaturvedi","sequence":"first","affiliation":[{"name":"Department of Biology, University of Texas at San Antonio  San Antonio TX USA"},{"name":"South Texas Center for Emerging Infectious Diseases University of Texas at San Antonio  San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan T.","family":"Weintraub","sequence":"additional","affiliation":[{"name":"Department of Biochemistry University of Texas Health Science Center at San Antonio  San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Lopez\u2010Ribot","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Texas at San Antonio  San Antonio TX USA"},{"name":"South Texas Center for Emerging Infectious Diseases University of Texas at San Antonio  San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Floyd L.","family":"Wormley","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Texas at San Antonio  San Antonio TX USA"},{"name":"South Texas Center for Emerging Infectious Diseases University of Texas at San Antonio  San Antonio TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198909213211205"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.4.624"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(86)90176-2"},{"key":"e_1_2_7_5_1","first-page":"502","article-title":"The pathology of pulmonary cryptococcal infections in the acquired immunodeficiency syndrome","volume":"110","author":"Gal A. 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