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Unfortunately, mass spectrometric analysis of the desired sequence fragments is often impeded due to low ionization efficiency and\/or signal suppression in complex samples. When several lysine residues are in close proximity tryptic peptides may be too short for mass analysis. Moreover, modified peptides often appear in low stoichiometry and need to be enriched before analysis. We present here how the use of sulfo\u2010NHS\u2010SS\u2010biotin derivatization of lysine side chain can help to detect PTMs in lysine\u2010rich proteins. This label leads to a mass shift which can be adjusted by reduction of the SS bridge and alkylation with different reagents. Low intensity peptides can be enriched by use of streptavidin beads. Using this method, the functionally relevant protein kinase A phosphorylation site in 5\u2010lipoxygenase was detected for the first time by MS. Additionally, methylation and acetylation could be unambiguously determined in histones.<\/jats:p>","DOI":"10.1002\/pmic.201300309","type":"journal-article","created":{"date-parts":[[2014,1,22]],"date-time":"2014-01-22T07:04:05Z","timestamp":1390374245000},"page":"659-667","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Sulfo\u2010NHS\u2010SS\u2010biotin derivatization: A versatile tool for <scp>MALDI<\/scp> mass analysis of PTMs in lysine\u2010rich proteins"],"prefix":"10.1002","volume":"14","author":[{"given":"Stavroula","family":"Markoutsa","sequence":"first","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ute","family":"Bahr","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios G.","family":"Papasotiriou","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann\u2010Kathrin","family":"H\u00e4fner","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Karas","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd L.","family":"Sorg","sequence":"additional","affiliation":[{"name":"Institute of Pharmaceutical Chemistry Goethe\u2010University  Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312568200"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-679X(10)98002-3"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700145"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mas.20219"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac103134e"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1200"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00726-010-0680-6"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.126813"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0803056105"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.2011.200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1522-2683(19991201)20:18<3551::AID-ELPS3551>3.0.CO;2-2"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.63.070194.002123"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2007.06.002"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111945200"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.01-0909fje"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2005.04.018"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015960"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.11.006"},{"key":"e_1_2_8_20_1","first-page":"351","article-title":"Histone acetylation and methylation: combinatorial players for transcriptional regulation","volume":"41","author":"An W.","year":"2007","journal-title":"Subcell. 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