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This pathogen can infect a wide range of hosts, and is the causative agent of Q fever in humans. Surface\u2010exposed and cell envelope associated proteins are thought to be important for both pathogenesis and protective immunity. Herein, we propose a complementary strategy consisting of (i) in silico prediction and (ii) inventory of the proteomic composition using three enrichment approaches coupled with protein identification. The efficiency of classical Triton X\u2010114 phase partitioning was compared with two novel procedures; isolation of alkaline proteins by liquid\u2010phase IEF, and cell surface enzymatic shaving using biofunctional magnetic beads. Of the 2026 protein sequences analyzed using seven distinct bioinformatic algorithms, 157 were predicted to be outer membrane proteins (OMP) and\/or lipoproteins (LP). Using the three enrichment protocols, we identified 196 nonredundant proteins, including 39 predicted OMP and\/or LP, 32 unknown or poorly characterized proteins, and 17 effectors of the Type IV secretion system. We additionally identified eight proteins with moonlighting activities, and several proteins apparently peripherally associated with integral or anchored OMP and\/or LP.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201300338","type":"journal-article","created":{"date-parts":[[2014,6,7]],"date-time":"2014-06-07T00:42:39Z","timestamp":1402101759000},"page":"1868-1881","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Identification of\n                    <i>Coxiella burnetii<\/i>\n                    surface\u2010exposed and cell envelope associated proteins using a combined bioinformatics plus proteomics strategy"],"prefix":"10.1002","volume":"14","author":[{"given":"Gabriela","family":"Flores\u2010Ramirez","sequence":"first","affiliation":[{"name":"Institute of Virology Slovak Academy of Sciences  Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbora","family":"Jankovicova","sequence":"additional","affiliation":[{"name":"Department of Biological and Biochemical Sciences Faculty of Chemical Technology, University of Pardubice  Pardubice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"Bilkova","sequence":"additional","affiliation":[{"name":"Department of Biological and Biochemical Sciences Faculty of Chemical Technology, University of Pardubice  Pardubice Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A.","family":"Miernyk","sequence":"additional","affiliation":[{"name":"USDA, Agricultural Research Service, Plant Genetics Research Unit, Department of Biochemistry University of Missouri  Columbia SC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ludovit","family":"Skultety","sequence":"additional","affiliation":[{"name":"Institute of Virology 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