{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:48:48Z","timestamp":1791406128806,"version":"4.3.3"},"reference-count":18,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T00:00:00Z","timestamp":1395273600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Swiss National Science Foundation","doi-asserted-by":"crossref","award":["31003A-132979"],"award-info":[{"award-number":["31003A-132979"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research and Technology Development","award":["51RT 0_126008"],"award-info":[{"award-number":["51RT 0_126008"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2014,5]]},"abstract":"\n AMP<\/jats:styled-content>\n ylation is a posttranslational modification (PTM) that has recently caught much attention in the context of bacterial infections as pathogens were shown to secrete\n F<\/jats:styled-content>\n ic proteins that\n AMP<\/jats:styled-content>\n ylate\n R<\/jats:styled-content>\n ho\n GTP<\/jats:styled-content>\n ases and thus interfere with host cell signaling processes. Although\n F<\/jats:styled-content>\n ic proteins are widespread and found in all kingdoms of life, only a small number of\n AMP<\/jats:styled-content>\n ylation targets are known to date. A major obstacle to target identification is the limited availability of generic strategies allowing sensitive and robust identification of\n AMP<\/jats:styled-content>\n ylation events. Here, we present an unbiased MS\u2010based approach utilizing stable isotope\u2010labeled\n ATP<\/jats:styled-content>\n . The\n ATP<\/jats:styled-content>\n isotopes are transferred onto target proteins in crude cell lysates by in vitro\n AMP<\/jats:styled-content>\n ylation introducing specific reporter ion clusters that allow detection of\n AMP<\/jats:styled-content>\n ylated peptides in complex biological samples by\n MS<\/jats:styled-content>\n analysis. Applying this strategy on the secreted\n F<\/jats:styled-content>\n ic protein\n B<\/jats:styled-content>\n ep2 of\n \n B<\/jats:styled-content>\n artonella rochalimae\n <\/jats:italic>\n , we identified the filamenting protein vimentin as an\n AMP<\/jats:styled-content>\n ylation target that was confirmed by independent assays. Vimentin represents a new class of target proteins and its identification emphasizes our method as a valuable tool to systematically uncover\n AMP<\/jats:styled-content>\n ylation targets. Furthermore, the approach can be generically adapted to study targets of other\n PTM<\/jats:styled-content>\n s that allow incorporation of isotopically labeled substrates.\n <\/jats:p>","DOI":"10.1002\/pmic.201300470","type":"journal-article","created":{"date-parts":[[2014,2,6]],"date-time":"2014-02-06T18:04:05Z","timestamp":1391709845000},"page":"1048-1052","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["An experimental strategy for the identification of\n AMP<\/scp>\n ylation targets from complex protein samples"],"prefix":"10.1002","volume":"14","author":[{"given":"Kathrin","family":"Pieles","sequence":"first","affiliation":[{"name":"Biozentrum University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Glatter","sequence":"additional","affiliation":[{"name":"Biozentrum University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Harms","sequence":"additional","affiliation":[{"name":"Biozentrum University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Schmidt","sequence":"additional","affiliation":[{"name":"Biozentrum University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Dehio","sequence":"additional","affiliation":[{"name":"Biozentrum University of Basel Basel Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,20]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.12.2949"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005818"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10729"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.262pe14"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1166382"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003142"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2009.03.008"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.227603"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2010.12.013"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja205137d"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201100743"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1280"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0808-748a"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr1011729"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001296"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05009-11"},{"key":"e_1_2_3_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406796102"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0910-681"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201300470","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201300470","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201300470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:12:01Z","timestamp":1761059521000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201300470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,20]]},"references-count":18,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2014,5]]}},"alternative-id":["10.1002\/pmic.201300470"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201300470","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,20]]},"assertion":[{"value":"2013-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}