{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:58:45Z","timestamp":1787317125634,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["C #23590703 to K.O."],"award-info":[{"award-number":["C #23590703 to K.O."]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["C #25462042 to M.T."],"award-info":[{"award-number":["C #25462042 to M.T."]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology of Japan to T.M"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:p>Exosomes are small vesicles secreted from cells that transport their embedded molecules through bidirectional exocytosis\u2010 and endocytosis\u2010like pathways. Expression patterns of exosomal molecules such as proteins and RNAs can be indicative of cell type since their signature is thought to be unique among cells. Using human primary (AZ\u2010521) and metastatic (AZ\u2010P7a) duodenal cancer cell lines, we conducted a comparative exosomal proteome analysis to identify proteins with metastatic marker potential. As determined by LC\u2010MS\/MS and Western blot analyses, polyadenylate\u2010binding protein 1 (PABP1) was found to be predominantly abundant in AZ\u2010P7a exosomes. The amount of exosomal PABP1 in AZ\u2010P7a cells increased by treating the cells with inhibitors for the classical ER\/Golgi secretory pathway (brefeldin A and monensin) and the ubiquitin\u2010proteasome pathway (MG\u2010132 and PYR\u201041). Treatment of AZ\u2010P7a cells with the neutral sphingomyelinase inhibitor GW4869, which suppresses exosome release, not only reduced the amount of exosomal PABP1 but also produced PABP1\u2010immunoreactive products cleaved via a proteolysis\u2010like process. Taken together, these results suggest that AZ\u2010P7a cells do not tolerate intracellular PABP1 accumulation and are thus exported into the extracellular milieu by the exosome\u2010mediated pathway. In addition, PABP1 has a potential use as a biomarker for metastatic duodenal cancer.<\/jats:p>","DOI":"10.1002\/pmic.201300477","type":"journal-article","created":{"date-parts":[[2014,7,27]],"date-time":"2014-07-27T05:19:27Z","timestamp":1406438367000},"page":"2297-2306","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Exosome\u2010mediated extracellular release of polyadenylate\u2010binding protein 1 in human metastatic duodenal cancer cells"],"prefix":"10.1002","volume":"14","author":[{"given":"Keiichi","family":"Ohshima","sequence":"first","affiliation":[{"name":"Medical Genetics Division Shizuoka Cancer Center Research Institute  Shizuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaori","family":"Kanto","sequence":"additional","affiliation":[{"name":"Medical Genetics Division Shizuoka Cancer Center Research Institute  Shizuoka 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