{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:48:31Z","timestamp":1789573711364,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,5,21]],"date-time":"2014-05-21T00:00:00Z","timestamp":1400630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Spanish National project","award":["AGL2009-2485-C03-02"],"award-info":[{"award-number":["AGL2009-2485-C03-02"]}]},{"name":"European Union project PARASITE","award":["FP7-312068"],"award-info":[{"award-number":["FP7-312068"]}]},{"name":"National Center for Biotechnology-CSIC"},{"name":"Spanish National Proteomics Institute","award":["2005\u00d7747_3"],"award-info":[{"award-number":["2005\u00d7747_3"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>The parasite species complex <jats:italic>Anisakis simplex sensu lato<\/jats:italic> (<jats:italic>Anisakis simplex sensu stricto<\/jats:italic>; (<jats:italic>A. simplex s.s<\/jats:italic>.), <jats:italic>A. pegreffii<\/jats:italic>, <jats:italic>A. simplex C<\/jats:italic>) is the main cause of severe anisakiasis (allergy) worldwide and is now an important health matter. In this study, the relationship of this <jats:italic>Anisakis<\/jats:italic> species complex and their allergenic capacities is assessed by studying the differences between the two most frequent species (<jats:italic>A. simplex s.s<\/jats:italic>., <jats:italic>A. pegreffii<\/jats:italic>) and their hybrid haplotype by studying active L3 larvae parasiting <jats:italic>Merluccius merluccius<\/jats:italic>. They were compared by 2D gel electrophoresis and parallel Western blot (2DE gels were hybridized with pools of sera from <jats:italic>Anisakis<\/jats:italic> allergenic patients). Unambiguous spot differences were detected and protein assignation was made by MALDI\u2010TOF\/TOF analysis or de novo sequencing. Seventy\u2010five gel spots were detected and the corresponding proteins were identified. Differentially expressed proteins for A<jats:italic>. simplex s.s., A. pegreffii<\/jats:italic>, and their hybrid are described and results are statistically supported. Twenty\u2010eight different allergenic proteins are classified according to different families belonging to different biological functions. These proteins are described for the first time as antigenic and potentially new allergens in <jats:italic>Anisakis<\/jats:italic>. Comparative proteomic analyses of allergenic capacities are useful for diagnosis, epidemiological surveys, and clinical research. All MS data have been deposited in the ProteomeXchange with identifier PXD000662 (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD000662\">http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD000662<\/jats:ext-link>).<\/jats:p>","DOI":"10.1002\/pmic.201300529","type":"journal-article","created":{"date-parts":[[2014,4,10]],"date-time":"2014-04-10T13:32:25Z","timestamp":1397136745000},"page":"1547-1568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Proteomic profiling and characterization of differential allergens in the nematodes <i>Anisakis simplex sensu stricto<\/i> and <i>A. pegreffii<\/i>"],"prefix":"10.1002","volume":"14","author":[{"given":"Susana C.","family":"Arcos","sequence":"first","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology Museo Nacional de Ciencias Naturales CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Ciordia","sequence":"additional","affiliation":[{"name":"Unidad de Proteomica Centro Nacional de Biotecnolog\u00eda CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee","family":"Roberston","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology Museo Nacional de Ciencias Naturales CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"In\u00e9s","family":"Zapico","sequence":"additional","affiliation":[{"name":"Unidad de Proteomica Centro Nacional de Biotecnolog\u00eda CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Jim\u00e9nez\u2010Ruiz","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology Museo Nacional de Ciencias Naturales CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Gonzalez\u2010Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Carlos III Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moneo","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Carlos III Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noelia","family":"Carballeda\u2010Sangiao","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Carlos III Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Rodriguez\u2010Mahillo","sequence":"additional","affiliation":[{"name":"Department of Immunology Hospital Carlos III Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan P.","family":"Albar","sequence":"additional","affiliation":[{"name":"Unidad de Proteomica Centro Nacional de Biotecnolog\u00eda CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Navas","sequence":"additional","affiliation":[{"name":"Department of Biodiversity and Evolutionary Biology Museo Nacional de Ciencias Naturales CSIC  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,5,21]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"97","article-title":"A nematode parasitic to herring causing acute abdominal syndromes in man","volume":"12","author":"Van Thiel P. 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