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This rare disease is considered as a leading cause of deaf\u2010blindness in humans worldwide. Cryosections of brain tissue were analyzed by MALDI\u2010MSI to differentiate between healthy and diseased rats. MSI results were highly reproducible. Tissue\u2010specific peptides were identified by MS\/MS using LC\u2010Orbitrap and MALDI\u2010TOF\/TOF analyses. These peptides were proposed for histological classification due to their particular spatial distribution in the brain, for example, substantia nigra, corpus callosum, and hippocampus. Several endogenous peptides showed significantly increased ion densities, particularly in the colliculi superiores and in the substantia nigra of diseased rats, including peptides derived from Fsd1, dystrobrevin\u2010\u03b2, and ProSAAS. Furthermore, several proteolytic degradation products of the myelin basic protein were identified, of which one peptide is most likely mediated by calpain\u20102. Our findings contribute to the characterization of this animal model and include possible peptide markers of disease.<\/jats:p>","DOI":"10.1002\/pmic.201300558","type":"journal-article","created":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T19:52:55Z","timestamp":1400529175000},"page":"1674-1687","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["MALDI imaging mass spectrometry to investigate endogenous peptides in an animal model of Usher's disease"],"prefix":"10.1002","volume":"14","author":[{"given":"Bijon","family":"Chatterji","sequence":"first","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa","family":"Dickhut","sequence":"additional","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Svenja","family":"Mielke","sequence":"additional","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonas","family":"Kr\u00fcger","sequence":"additional","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ingo","family":"Just","sequence":"additional","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silke","family":"Glage","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Science Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Meier","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Science Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Wedekind","sequence":"additional","affiliation":[{"name":"Institute of Laboratory Animal Science Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Pich","sequence":"additional","affiliation":[{"name":"Institute of Toxicology Hannover Medical School  Hannover Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32834ef8b2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-006-0304-0"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0015669"},{"key":"e_1_2_8_5_1","first-page":"495","article-title":"Sequence variants of the DFNB31 gene among Usher syndrome patients of diverse origin","volume":"16","author":"Aller E.","year":"2010","journal-title":"Mol. 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