{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T05:32:57Z","timestamp":1776403977453,"version":"3.51.2"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Program for Basic Research of China","award":["2012CB910603"],"award-info":[{"award-number":["2012CB910603"]}]},{"name":"State Key Laboratory of Space Medicine Fundamentals and Application, Chinese Astronaut Research and Training Center","award":["SMFA10K05"],"award-info":[{"award-number":["SMFA10K05"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>Microgravity severely halts the structural and functional cerebral capacity of astronauts especially affecting their brains due to the stress produced by cephalic fluid shift. We employed a rat tail suspension model to substantiate simulated microgravity (SM) in brain. In this study, comparative mass spectrometry was applied in order to demonstrate the differential expression of 17 specific cellular defense proteins. Gamma\u2010enolase, peptidyl\u2010prolyl cis\u2010trans isomerase A, glial fibrillary acidic protein, heat shock protein HSP 90\u2010alpha, 10 kDa heat shock protein, mitochondrial, heat shock cognate 71 kDa protein, superoxide dismutase 1 and dihydropyrimidinase\u2010related protein 2 were found to be upregulated by HPLC\/ESI\u2010TOF. Furthermore, five differentially expressed proteins including 60 kDa heat shock protein, mitochondrial, heat shock protein HSP 90\u2010beta, peroxiredoxin\u20102, stress\u2010induced\u2010phosphoprotein, and UCHL\u20101 were found to be upregulated by HPLC\/ESI\u2010Q\u2010TOF MS. In addition, downregulated proteins include cytochrome C, superoxide dismutase 2, somatic, and excitatory amino acid transporter 1 and protein DJ\u20101. Validity of MS results was successfully performed by Western blot analysis of DJ\u20101 protein. This study will not only help to understand the neurochemical responses produced under microgravity but also will give future direction to cure the proteomic losses and their after effects in astronauts.<\/jats:p>","DOI":"10.1002\/pmic.201400019","type":"journal-article","created":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T06:38:45Z","timestamp":1395297525000},"page":"1424-1433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Differential expression of specific cellular defense proteins in rat hypothalamus under simulated microgravity induced conditions: Comparative proteomics"],"prefix":"10.1002","volume":"14","author":[{"given":"Javed","family":"Iqbal","sequence":"first","affiliation":[{"name":"School of Life Sciences Beijing Institute of Technology  Beijing P. R. 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