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Cardiac resynchronization therapy (CRT) can reverse ventricular remodeling and improve cardiac function. Although the underlying molecular changes are unknown, the use of a canine model of dyssynchronous heart failure (DHF) and CRT has shown that there are global changes across the cardiac proteome. This study determines changes in serum glycoprotein concentration from DHF and CRT compared to normal. We hypothesize that CRT invokes protective or advantageous pathways that can be reflected in the circulating proteome. Two prong discovery approaches were carried out on pooled normal, DHF, and CRT samples composed of individual canine serum to determine the overall protein concentration and the <jats:italic>N<\/jats:italic>\u2010linked glycosites of circulating glycoproteins. The level of the glycoproteins was altered in DHF and CRT compared to control sera, with 63 glycopeptides substantially increased in DHF and\/or CRT. Among the 32 elevated glycosite\u2010containing peptides in DHF, 13 glycopeptides were reverted to normal level after CRT therapy. We further verify the changes of glycopeptides using label\u2010free LC\u2010MS from individual canine serum. Circulating glycoproteins such as alpha\u2010fetoprotein, alpha\u20102\u2010macroglobulin, galectin\u20103\u2010binding protein, and collectin\u201010 show association to failing heart and CRT treatment model.<\/jats:p>","DOI":"10.1002\/pmic.201400151","type":"journal-article","created":{"date-parts":[[2014,8,20]],"date-time":"2014-08-20T21:45:13Z","timestamp":1408571113000},"page":"567-579","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Glycoproteins identified from heart failure and treatment models"],"prefix":"10.1002","volume":"15","author":[{"given":"Shuang","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shisheng","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punit","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiming","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Kass","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Medicine Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"van Eyk","sequence":"additional","affiliation":[{"name":"Department of Medicine Johns Hopkins University  Baltimore MD USA"},{"name":"Advanced Clinical Biosystems Research Institute Cedar Sinai Medical Center  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Johns Hopkins University  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,10,9]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"1","article-title":"National vital statistics reports","volume":"59","author":"Kochanek K. 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