{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T17:56:08Z","timestamp":1775411768116,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,3,16]],"date-time":"2015-03-16T00:00:00Z","timestamp":1426464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Science Foundation of China","doi-asserted-by":"crossref","award":["Grant No. 81202424"],"award-info":[{"award-number":["Grant No. 81202424"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Research Project of the Chinese Ministry of Education","award":["No.113037A"],"award-info":[{"award-number":["No.113037A"]}]},{"name":"Zhejiang Provincial Natural Science Foundation of China","award":["Grant No. LZ14H280001"],"award-info":[{"award-number":["Grant No. LZ14H280001"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:p><jats:italic>Ginkgo biloba<\/jats:italic> is an attractive and traditional medicinal plant, and has been widely used as a phytomedicine in the prevention and treatment of cardiovascular and cerebrovascular diseases. Flavonoids and terpene lactones are the major bioactive components of <jats:italic>Ginkgo<\/jats:italic>, whereas the ginkgolic acids (GAs) with strong allergenic properties are strictly controlled. In this study, we tested the content of flavonoids and GAs under ultraviolet\u2010B (UV\u2010B) treatment and performed comparative proteomic analyses to determine the differential proteins that occur upon UV\u2010B radiation. That might play a crucial\u00a0role in producing flavonoids and GAs. Our phytochemical analyses demonstrated that UV\u2010B irradiation significantly increased the content of active flavonoids, and decreased the content of toxic GAs. We conducted comparative proteomic analysis of both whole leaf and chloroplasts proteins. In total, 27 differential proteins in the whole leaf and 43 differential proteins in the chloroplast were positively identified and functionally annotated. The proteomic data suggested that enhanced UV\u2010B radiation exposure activated antioxidants and stress\u2010responsive proteins as well as reduced the rate of photosynthesis. We demonstrate that UV\u2010B irradiation pharmaceutically improved the metabolic ingredients of <jats:italic>Ginkgo<\/jats:italic>, particularly in terms of reducing GAs. With high UV absorption properties, and antioxidant activities, the flavonoids were likely highly induced as protective molecules following UV\u2010B irradiation.<\/jats:p>","DOI":"10.1002\/pmic.201400258","type":"journal-article","created":{"date-parts":[[2015,1,20]],"date-time":"2015-01-20T09:11:38Z","timestamp":1421745098000},"page":"1868-1883","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Improved metabolites of pharmaceutical ingredient grade <i>Ginkgo biloba<\/i> and the correlated proteomics analysis"],"prefix":"10.1002","volume":"15","author":[{"given":"Wen","family":"Zheng","sequence":"first","affiliation":[{"name":"College of Biomedical Engineering &amp; Instrument Science Zhejiang University  Hangzhou Zhejiang P.\u00a0R. 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