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In this study, comparative tissue proteomic analysis of low and high grades of MGs was performed by using iTRAQ\u2010based quantitative proteomics in combination with ESI\u2010quadrupole\u2010TOF and Q\u2010Exactive MS, and results were validated by employing ELISA. Combining the results obtained from two MS platforms, we were able to identify overall 4308 proteins (1% false discover rate), among which 2367 exhibited differential expression (more than and equal to 2 peptide and \u22651.5\u2010fold in at least one grade) in MGs. Several differentially expressed proteins were found to be associated with diverse signaling pathways, including integrin, Wnt, Ras, epidermal growth factor receptor, and FGR signaling. Proteins, such as vinculin or histones, which act as the signaling activators to initiate multiple signaling pathways, were found to be upregulated in MGs. Quite a few candidates, such as protein S\u2010100A6, aldehyde dehydrogenase mitochondrial, AHNAK, cytoskeleton\u2010associated protein 4, and caveolin, showed sequential increase in low\u2010 and high\u2010grade MGs, whereas differential expressions of collagen alpha\u20101 (VI), protein S100\u2010A9, 14 kDa phosphohistidine phosphatase, or transgelin\u20102 were found to be grade specific. Our findings provide new insights regarding the association of various signal transduction pathways in MG pathogenesis and may introduce new opportunities for the early detection and prognosis of MGs.<\/jats:p>","DOI":"10.1002\/pmic.201400328","type":"journal-article","created":{"date-parts":[[2014,11,21]],"date-time":"2014-11-21T02:00:48Z","timestamp":1416535248000},"page":"394-407","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Multipronged quantitative proteomic analyses indicate modulation of various signal transduction pathways in human meningiomas"],"prefix":"10.1002","volume":"15","author":[{"given":"Samridhi","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandipan","family":"Ray","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuvolina","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aliasgar","family":"Moiyadi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Advanced Center for Treatment Research and Education in Cancer Tata Memorial Center  Kharghar Navi Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Epari","family":"Sridhar","sequence":"additional","affiliation":[{"name":"Department of Pathology Tata Memorial Hospital  Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeeva","family":"Srivastava","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Bioengineering Indian Institute of Technology Bombay  Powai Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,15]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"813","article-title":"Non\u2010glial tumors of the brain: tumors of the cerebellopontine angle and meningiomas","volume":"5","author":"Long D. 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