{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T22:56:15Z","timestamp":1791413775461,"version":"4.3.3"},"reference-count":28,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2015,8,17]],"date-time":"2015-08-17T00:00:00Z","timestamp":1439769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001866","name":"Fonds National de la Recherche Luxembourg","doi-asserted-by":"publisher","award":["CPIL"],"award-info":[{"award-number":["CPIL"]}],"id":[{"id":"10.13039\/501100001866","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>The quantification of plasma proteins using the high resolution and accurate mass (HR\/AM)\u2010based parallel reaction monitoring (PRM) method provides an immediate benefit over the conventional SRM\u2010based method in terms of selectivity. In this study, multiplexed PRM assays were developed to analyze isotypes of serum amyloid A (SAA) proteins in human plasma with a focus on SAA1 and SAA2. Elevated plasma levels of these proteins in patients diagnosed with lung cancer have been reported in previous studies. Since SAA1 and SAA2 are highly homologous, the available immunoassays tend to overestimate their concentrations due to cross\u2010reactivity. On the other hand, when mass spectrometry (MS)\u2010based assays are used, the presence of the several allelic variants may result in a problem of underestimation. In the present study, eight peptides that represent the target proteins at three different levels: isotype\u2010specific (SAA1\u03b1,\u00a0 SAA 1\u03b2,\u00a0 SAA1\u03b3,\u00a0 SAA2\u03b1,\u00a0 SAA2\u03b2), protein\u2010specific (SAA1 or SAA2), and pan SAA (SAA1 and SAA2) were chosen to differentiate SAAs in lung cancer plasma samples using a panel of PRM assays. The measurement of specific isotypes, leveraging the analytical performance of PRM, allowed to quantify the allelic variants of both target proteins. The isotypes detected were corroborated with the genetic information obtained from the same samples. The combination of SAA2\u03b1\u00a0and SAA2\u03b2\u00a0assays representing the total SAA2 concentration demonstrated a superior analytical outcome than the previously used assay on the common peptide when applied to the detection of lung cancer.<\/jats:p>","DOI":"10.1002\/pmic.201400382","type":"journal-article","created":{"date-parts":[[2015,7,16]],"date-time":"2015-07-16T05:44:28Z","timestamp":1437025468000},"page":"3116-3125","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":64,"title":["Quantification of SAA1 and SAA2 in lung cancer plasma using the isotype\u2010specific PRM assays"],"prefix":"10.1002","volume":"15","author":[{"given":"Yeoun Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Luxembourg Clinical Proteomics Center Luxembourg Institute of Health  Strassen 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