{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T14:40:42Z","timestamp":1791124842652,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,1,14]],"date-time":"2015-01-14T00:00:00Z","timestamp":1421193600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 EY024596, R01 AG027012"],"award-info":[{"award-number":["R01 EY024596, R01 AG027012"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p>The retina is a delicate tissue that detects light, converts photochemical energy into neural signals, and transmits the signals to the visual cortex of the brain. A detailed protein inventory of the proteome of the normal human eye may provide a foundation for new investigations into both the physiology of the retina and the pathophysiology of retinal diseases. To provide an inventory, proteins were extracted from five retinas of normal eyes and fractionated using SDS\u2010PAGE. After in\u2010gel digestion, peptides were analyzed in duplicate using LC\u2013MS\/MS on an Orbitrap Elite mass spectrometer. A total of 3436 nonredundant proteins were identified in the human retina, including 20 unambiguous protein isoforms, of which eight have not previously been demonstrated to exist at the protein level. The proteins identified in the retina included most of the enzymes involved in the visual cycle and retinoid metabolism. One hundred and fifty\u2010eight proteins that have been associated with age\u2010related macular degeneration were identified in the retina. The MS proteome database of the human retina may serve as a valuable resource for future investigations of retinal biology and disease. All MS data have been deposited in the ProteomeXchange with identifier PXD001242 (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD001242\">http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD001242<\/jats:ext-link>).<\/jats:p>","DOI":"10.1002\/pmic.201400397","type":"journal-article","created":{"date-parts":[[2014,11,19]],"date-time":"2014-11-19T04:54:50Z","timestamp":1416372890000},"page":"836-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["The proteome of human retina"],"prefix":"10.1002","volume":"15","author":[{"given":"Pingbo","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  West Palm Beach FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randi","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ferri","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"},{"name":"Rochester Eye Associates  Rochester NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidya","family":"Venkatraman","sequence":"additional","affiliation":[{"name":"Advanced Clinical BioSystems Research Institute, The Heart Institute and Department of Medicine Cedars\u2010Sinai Medical Center  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia","family":"Karani","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard A.","family":"Lutty","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"Van Eyk","sequence":"additional","affiliation":[{"name":"Advanced Clinical BioSystems Research Institute, The Heart Institute and Department of Medicine Cedars\u2010Sinai Medical Center  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Semba","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0801537"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1005073"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0804630"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/192582"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopthalmol.2011.1427"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138920211795860107"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300075"},{"key":"e_1_2_5_9_1","first-page":"e2455","article-title":"Dissection of human vitreous body elements for proteomic analysis","volume":"47","author":"Skeie J. 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