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Rho\u2010kinase inhibitor Y\u201027632 (ROCKi) is a potential drug molecule, which has been reported to support the gene expressions typical for the chondrocytes, thus restricting their phenotypic conversion to fibroblastic cells upon the cellular expansion. In this study, we have investigated the short\u2010term biological responses of ROCKi to human primary foreskin fibroblasts. The fibroblast cells were exposed to 1 and 10 \u03bcM ROCKi treatments. A proteomics analysis revealed expression changes of 56 proteins, and a further protein pathway analysis suggested their association with the cell morphology, the organization, and the increased cellular movement and the proliferation. These functional responses were confirmed by a Cell\u2010IQ time\u2010lapse imaging analysis. Rho\u2010kinase inhibitor treatment increased the cellular proliferation up to twofold during the first 12 h, and a wound model based migration assay showed 50% faster filling of the mechanically generated wound area. Additionally, significantly less vinculin\u2010associated focal adhesions were present in the ROCKi\u2010treated cells. Despite the marked changes in the cell behavior, ROCKi was not able to induce the expression of the chondrocyte\u2010specific genes, such as procollagen \u03b1<jats:sub>1<\/jats:sub>(II) and aggrecan.<\/jats:p>","DOI":"10.1002\/pmic.201400417","type":"journal-article","created":{"date-parts":[[2015,5,6]],"date-time":"2015-05-06T02:09:28Z","timestamp":1430878168000},"page":"2953-2965","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Rho\u2010kinase inhibitor Y\u201027632 increases cellular proliferation and migration in human foreskin fibroblast cells"],"prefix":"10.1002","volume":"15","author":[{"given":"Juha","family":"Piltti","sequence":"first","affiliation":[{"name":"Institute of Biomedicine University of Eastern Finland  Kuopio Finland"},{"name":"Department of Integrative Medical Biology University of Ume\u00e5  Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markku","family":"Varjosalo","sequence":"additional","affiliation":[{"name":"Institute of Biotechnology University of Helsinki  Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengjuan","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of Applied Physics University of Eastern Finland  Kuopio Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jukka","family":"H\u00e4yrinen","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine University of Eastern Finland  Kuopio Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikko J.","family":"Lammi","sequence":"additional","affiliation":[{"name":"Department of Integrative Medical Biology University of Ume\u00e5  Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.07.024"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1151526"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.11.019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2013.05.029"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/267531a0"},{"key":"e_1_2_7_7_1","first-page":"467","article-title":"Development and phenotypic characterization of a high density in vitro model of auricular chondrocytes with applications in reconstructive plastic surgery","volume":"21","author":"Haisch A.","year":"2006","journal-title":"Histol. 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