{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T20:37:44Z","timestamp":1781901464237,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,3,18]],"date-time":"2015-03-18T00:00:00Z","timestamp":1426636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Institute of Biomedical Innovation of Japan"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:p>Lumbar spinal stenosis (LSS) is a syndromic degenerative spinal disease and is characterized by spinal canal narrowing with subsequent neural compression causing gait disturbances. Although LSS is a major age\u2010related musculoskeletal disease that causes large decreases in the daily living activities of the elderly, its molecular pathology has not been investigated using proteomics. Thus, we used several proteomic technologies to analyze the ligamentum flavum (LF) of individuals with LSS. Using comprehensive proteomics with strong cation exchange fractionation, we detected 1288 proteins in these LF samples. A GO analysis of the comprehensive proteome revealed that more than 30% of the identified proteins were extracellular. Next, we used 2D image converted analysis of LC\/MS to compare LF obtained from individuals with LSS to that obtained from individuals with disc herniation (nondegenerative control). We detected 64\u00a0781 MS peaks and identified 1675 differentially expressed peptides derived from 286 proteins. We verified four differentially expressed proteins (fibronectin, serine protease HTRA1, tenascin, and asporin) by quantitative proteomics using SRM\/MRM. The present proteomic study is the first to identify proteins from degenerated and hypertrophied LF in LSS, which will help in studying LSS.<\/jats:p>","DOI":"10.1002\/pmic.201400442","type":"journal-article","created":{"date-parts":[[2015,1,12]],"date-time":"2015-01-12T08:17:34Z","timestamp":1421050654000},"page":"1622-1630","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Proteomic analysis of ligamentum flavum from patients with lumbar spinal stenosis"],"prefix":"10.1002","volume":"15","author":[{"given":"Masahiro","family":"Kamita","sequence":"first","affiliation":[{"name":"Division of Chemotherapy and Clinical Research National Cancer Center Research Institute  Tsukiji Chuo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taiki","family":"Mori","sequence":"additional","affiliation":[{"name":"BioBank Omics Unit National Center for Geriatrics and Gerontology (NCGG)  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihito","family":"Sakai","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery NCGG  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadayuki","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery NCGG  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Gomi","sequence":"additional","affiliation":[{"name":"BioBusiness Group Mitsui Knowledge Industry  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy and Clinical Research National Cancer Center Research Institute  Tsukiji Chuo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery NCGG  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumpei","family":"Niida","sequence":"additional","affiliation":[{"name":"BioBank Omics Unit National Center for Geriatrics and Gerontology (NCGG)  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tesshi","family":"Yamada","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy and Clinical Research National Cancer Center Research Institute  Tsukiji Chuo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Bone and Joint Disease NCGG  Morioka, Obu, Aichi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Ono","sequence":"additional","affiliation":[{"name":"Division of Chemotherapy and Clinical Research National Cancer Center Research Institute  Tsukiji Chuo\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp0708097"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2009.90"},{"key":"e_1_2_8_4_1","unstructured":"North American Spine Society 2011 NASS evidence\u2010based clinical guidelines committee: diagnosis and treatment of degenerative lumbar spinal stenosis\u2014evidence\u2010based clinical guidelines for multidisciplinary spine care"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1097\/01.BRS.0000049927.37696.DC","article-title":"Rates of advanced spinal imaging and spine surgery","volume":"28","author":"Lurie J. 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