{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T19:35:07Z","timestamp":1789414507226,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2015,9,22]],"date-time":"2015-09-22T00:00:00Z","timestamp":1442880000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MICROGEN","award":["CSD2009-00006 and 2012-40007"],"award-info":[{"award-number":["CSD2009-00006 and 2012-40007"]}]},{"name":"Spanish Ministry of Economy and Competitiveness"},{"name":"Center for Analytical Biosciences","award":["61310-0110-13761"],"award-info":[{"award-number":["61310-0110-13761"]}]},{"name":"Center for Epigenetics","award":["62310-0112-023541"],"award-info":[{"award-number":["62310-0112-023541"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:p>\n                    Tooth decay is considered the most prevalent human disease worldwide. We present the first metaproteomic study of the oral biofilm, using different mass spectrometry approaches that have allowed us to quantify individual peptides in healthy and caries\u2010bearing individuals. A total of 7771 bacterial and 853 human proteins were identified in 17 individuals, which provide the first available protein repertoire of human dental plaque.\n                    <jats:italic>Actinomyces<\/jats:italic>\n                    and\n                    <jats:italic>Coryneybacterium<\/jats:italic>\n                    represent a large proportion of the protein activity followed by\n                    <jats:italic>Rothia<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    . Those four genera account for 60\u201390% of total diversity. Healthy individuals appeared to have significantly higher amounts of L\u2010lactate dehydrogenase and the arginine deiminase system, both implicated in pH buffering. Other proteins found to be at significantly higher levels in healthy individuals were involved in exopolysaccharide synthesis, iron metabolism and immune response. We applied multivariate analysis in order to find the minimum set of proteins that better allows discrimination of healthy and caries\u2010affected dental plaque samples, detecting seven bacterial and five human protein functions that allow determining the health status of the studied individuals with an estimated specificity and sensitivity over 96%. We propose that future validation of these potential biomarkers in larger sample size studies may serve to develop diagnostic tests of caries risk that could be used in tooth decay prevention.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201400600","type":"journal-article","created":{"date-parts":[[2015,8,14]],"date-time":"2015-08-14T05:23:23Z","timestamp":1439529803000},"page":"3497-3507","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":79,"title":["The human oral metaproteome reveals potential biomarkers for caries disease"],"prefix":"10.1002","volume":"15","author":[{"given":"Pedro","family":"Belda\u2010Ferre","sequence":"first","affiliation":[{"name":"FISABIO Foundation Center for Advanced Research in Public Health  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Williamson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Southern Denmark  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1urea","family":"Sim\u00f3n\u2010Soro","sequence":"additional","affiliation":[{"name":"FISABIO Foundation Center for Advanced Research in Public Health  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Artacho","sequence":"additional","affiliation":[{"name":"FISABIO Foundation Center for Advanced Research in Public Health  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ole N.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology University of Southern Denmark  Odense Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex","family":"Mira","sequence":"additional","affiliation":[{"name":"FISABIO Foundation Center for Advanced Research in Public Health  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11209"},{"key":"e_1_2_8_3_1","first-page":"318","volume-title":"Microb. 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