{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T18:17:22Z","timestamp":1771265842104,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T00:00:00Z","timestamp":1441584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Science Foundation Major Research Instrumentation","award":["0923536"],"award-info":[{"award-number":["0923536"]}]},{"name":"American Recovery and Reinvestment Act","award":["RC2 HG005805"],"award-info":[{"award-number":["RC2 HG005805"]}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"crossref","award":["S10 RR027584"],"award-info":[{"award-number":["S10 RR027584"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"crossref","award":["R01 GM087221"],"award-info":[{"award-number":["R01 GM087221"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p><jats:italic>Staphylococcus aureus<\/jats:italic> is an opportunistic human pathogen, which can cause life\u2010threatening disease. Proteome analyses of the bacterium can provide new insights into its pathophysiology and important facets of metabolic adaptation and, thus, aid the recognition of targets for intervention. However, the value of such proteome studies increases with their comprehensiveness. We present an MS\u2013driven, proteome\u2010wide characterization of the strain <jats:italic>S. aureus<\/jats:italic> HG001. Combining 144 high precision proteomic data sets, we identified 19 109 peptides from 2088 distinct <jats:italic>S. aureus<\/jats:italic> HG001 proteins, which account for 72% of the predicted ORFs. Peptides were further characterized concerning p<jats:italic>I<\/jats:italic>, GRAVY, and detectability scores in order to understand the low peptide coverage of 8.7% (19 109 out of 220 245 theoretical peptides). The high quality peptide\u2010centric spectra have been organized into a comprehensive peptide fragmentation library (SpectraST) and used for identification of <jats:italic>S. aureus<\/jats:italic>\u2010typic peptides in highly complex host\u2013pathogen interaction experiments, which significantly improved the number of identified <jats:italic>S. aureus<\/jats:italic> proteins compared to a MASCOT search. This effort now allows the elucidation of crucial pathophysiological questions in <jats:italic>S. aureus<\/jats:italic>\u2010specific host\u2013pathogen interaction studies through comprehensive proteome analysis. The <jats:italic>S. aureus<\/jats:italic>\u2010specific spectra resource developed here also represents an important spectral repository for SRM or for data\u2010independent acquisition MS approaches. All MS data have been deposited in the ProteomeXchange with identifier PXD000702 (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD000702\">http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD000702<\/jats:ext-link>).<\/jats:p>","DOI":"10.1002\/pmic.201500091","type":"journal-article","created":{"date-parts":[[2015,7,30]],"date-time":"2015-07-30T05:49:09Z","timestamp":1438235349000},"page":"3648-3661","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["A peptide resource for the analysis of <i>Staphylococcus aureus<\/i> in host\u2013pathogen interaction studies"],"prefix":"10.1002","volume":"15","author":[{"given":"Maren","family":"Depke","sequence":"first","affiliation":[{"name":"ZIK\u2010FunGene Junior Research Group \u201cApplied Proteomics\u201d Interfaculty Institute for Genetics and Functional Genomics Department of 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