{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T03:20:13Z","timestamp":1762917613517,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2015,10,16]],"date-time":"2015-10-16T00:00:00Z","timestamp":1444953600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p>Enterovirus 71 (EV71) is one of the leading causes of hand, foot and mouth disease with neurological complications in some cases. To study the pathogenesis of EV71 infection, large\u2010scale analyses of EV71 infected cells have been performed. However, most of these studies employed rhabdomyosarcoma (RD) cells or used transcriptomic strategy. Here, we performed SILAC\u2010based quantitative proteomic analysis of EV71\u2010infected U251 cells, a human glioma cell line. A total of 3125 host proteins were quantified, in which 451 were differentially regulated as a result of EV71 infection at 8 or 20 hpi or both. Gene Ontology analysis indicates the regulated proteins were enriched in \u201cmetabolic process\u201d, \u201cbiological regulation\u201d and \u201ccellular process\u201d, implying that these biological processes were affected by EV71 infection. Furthermore, functional study indicated that TRAF2 and TRAF6 among the up\u2010regulated proteins could inhibit the replication of EV71 at the early phase post infection, and the anti\u2010EV71 function of both proteins was independent of interferon \u03b2. Our study not only provided an overview of cellular response to EV71 infection in a human glioma cell line, but also found that TRAF2 and TRAF6 might be potential targets to inhibit the replication of EV71. All MS data have been deposited in the ProteomeXchange with identifier PXD002454 (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD002454\">http:\/\/proteomecentral.proteomexchange.org\/dataset\/PXD002454<\/jats:ext-link>).<\/jats:p>","DOI":"10.1002\/pmic.201500134","type":"journal-article","created":{"date-parts":[[2015,9,9]],"date-time":"2015-09-09T07:30:56Z","timestamp":1441783856000},"page":"3784-3796","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Global quantitative proteomic analysis of human glioma cells profiled host protein expression in response to enterovirus type 71 infection"],"prefix":"10.1002","volume":"15","author":[{"given":"Lei\u2010Ke","family":"Zhang","sequence":"first","affiliation":[{"name":"Wuhan Institute of Virology Chinese Academy of Sciences  Wuhan P. R. 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