{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T11:42:38Z","timestamp":1791459758833,"version":"4.3.4"},"reference-count":66,"publisher":"Wiley","issue":"23-24","license":[{"start":{"date-parts":[[2015,9,25]],"date-time":"2015-09-25T00:00:00Z","timestamp":1443139200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:p>\n                    Sperm proteins mediating sperm\u2013egg interaction should be exhibited on the sperm surface, or exposed or released when sperm approach an egg. In ascidians (protochordates), sperm undergo a sperm reaction, characterized by enhanced sperm motility and mitochondrial swelling and shedding on contact with the vitelline coat (VC) or by treatment with Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ionophore. Here, proteomic analysis was conducted on sperm exudates and sperm surface proteins using ionomycin\u2010induced sperm reaction and cell\u2010impermeable labeling in\n                    <jats:italic>Ciona intestinalis<\/jats:italic>\n                    type A (\n                    <jats:italic>C. robusta<\/jats:italic>\n                    ). In the exudate from sperm treated with ionomycin, membrane proteins including a possible VC receptor CiUrabin were abundant, indicating the release of membranous compartments during sperm reaction. Among the surface proteins XP_009859314.1 (uncharacterized protein exhibiting homology to HrTTSP\u20101) was most abundant before the sperm reaction, but XP_004227079.1 (unknown Ig superfamily protein) appears to be most abundantly exposed by the sperm reaction. Moreover, proteins containing a notable set of domains, astacin\u2010like metalloprotease domain and thrombospondin type 1 repeat(s), were found in this fraction. Possible roles in fertilization as well as localizations and behaviors of these proteins are discussed.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201500162","type":"journal-article","created":{"date-parts":[[2015,7,29]],"date-time":"2015-07-29T06:31:23Z","timestamp":1438151483000},"page":"4064-4079","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteomics of ionomycin\u2010induced ascidian sperm reaction: Released and exposed sperm proteins in the ascidian\n                    <i>Ciona intestinalis<\/i>"],"prefix":"10.1002","volume":"15","author":[{"given":"Shiori","family":"Nakazawa","sequence":"first","affiliation":[{"name":"Sugashima Marine Biological Laboratory Graduate School of Science Nagoya University  Sugashima Toba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Shirae\u2010Kurabayashi","sequence":"additional","affiliation":[{"name":"Sugashima Marine Biological Laboratory Graduate School of Science Nagoya University  Sugashima Toba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Otsuka","sequence":"additional","affiliation":[{"name":"Sugashima Marine Biological Laboratory Graduate School of Science Nagoya University  Sugashima Toba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Sawada","sequence":"additional","affiliation":[{"name":"Sugashima Marine Biological Laboratory Graduate School of Science Nagoya University  Sugashima Toba Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,25]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Biology of Fertilization","author":"Metz C. 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