{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:27:09Z","timestamp":1792711629047,"version":"4.5.0"},"reference-count":11,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,3,11]],"date-time":"2016-03-11T00:00:00Z","timestamp":1457654400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:p>The iris is a fine structure that controls the amount of light that enters the eye. The ciliary body controls the shape of the lens and produces aqueous humor. The retinal pigment epithelium and choroid (RPE\/choroid) are essential in supporting the retina and absorbing light energy that enters the eye. Proteins were extracted from iris, ciliary body, and RPE\/choroid tissues of eyes from five individuals and fractionated using SDS\u2010PAGE. After in\u2010gel digestion, peptides were analyzed using LC\u2010MS\/MS on an Orbitrap Elite mass spectrometer. In iris, ciliary body, and RPE\/choroid, we identified 2959, 2867, and 2755 nonredundant proteins with peptide and protein false\u2010positive rates of &lt;0.1% and &lt;1%, respectively. Forty\u2010three unambiguous protein isoforms were identified in iris, ciliary body, and RPE\/choroid. Four \u201cmissing proteins\u201d were identified in ciliary body based on \u22652 proteotypic peptides. The mass spectrometric proteome database of the human iris, ciliary body, and RPE\/choroid may serve as a valuable resource for future investigations of the eye in health and disease. The MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD001424 and PXD002194.<\/jats:p>","DOI":"10.1002\/pmic.201500188","type":"journal-article","created":{"date-parts":[[2016,2,2]],"date-time":"2016-02-02T03:32:58Z","timestamp":1454383978000},"page":"1146-1153","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Defining the proteome of human iris, ciliary body, retinal pigment epithelium, and choroid"],"prefix":"10.1002","volume":"16","author":[{"given":"Pingbo","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Kirby","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Dufresne","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  West Palm Beach FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Randi","family":"Turner","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Ferri","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepak P.","family":"Edward","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"},{"name":"King Khaled Eye Specialist Hospital  Riyadh Kingdom of Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer E.","family":"Van Eyk","sequence":"additional","affiliation":[{"name":"Advanced Clinical Biosystems Research Institute Cedars\u2010Sinai Medical Center  Los Angeles CA USA"},{"name":"Department of Medicine The Heart Institute, Cedars\u2010Sinai Medical Center  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard D.","family":"Semba","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Johns Hopkins University School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00021.2004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201300075"},{"key":"e_1_2_6_4_1","first-page":"e2455","article-title":"Dissection of human vitreous body elements for proteomic analysis","volume":"47","author":"Skeie J. 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