{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:44:03Z","timestamp":1762101843061,"version":"build-2065373602"},"reference-count":53,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,13]],"date-time":"2016-04-13T00:00:00Z","timestamp":1460505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003093","name":"Malaysia Ministry of Higher Education","doi-asserted-by":"crossref","award":["E000013-20001"],"award-info":[{"award-number":["E000013-20001"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Grant Scheme","award":["FRGS, FP030-2014A"],"award-info":[{"award-number":["FRGS, FP030-2014A"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic> is the leading causative agent of bacterial sexually transmitted infections worldwide which can lead to female pelvic inflammatory disease and infertility. A greater understanding of host response during chlamydial infection is essential to design intervention technique to reduce the increasing incidence rate of genital chlamydial infection. In this study, we investigated proteome changes in epithelial cells during <jats:italic>C. trachomatis<\/jats:italic> infection by using an isobaric tags for relative and absolute quantitation (iTRAQ) labeling technique coupled with a liquid chromatography\u2010tandem mass spectrometry (LC\u2010MS<jats:sup>3<\/jats:sup>) analysis. <jats:italic>C. trachomatis<\/jats:italic> (serovar D, MOI 1)\u2013infected HeLa\u2010229 human cervical carcinoma epithelial cells (at 2, 4 and 8 h) showed profound modifications of proteome profile which involved 606 host proteins. MGST1, SUGP2 and ATXN10 were among the top in the list of the differentially upregulated protein. Through pathway analysis, we suggested the involvement of eukaryotic initiation factor 2 (eIF2) and mammalian target of rapamycin (mTOR) in host cells upon <jats:italic>C. trachomatis<\/jats:italic> infection. Network analysis underscored the participation of DNA repair mechanism during <jats:italic>C. trachomatis<\/jats:italic> infection. In summary, intense modifications of proteome profile in <jats:italic>C. trachomatis<\/jats:italic>\u2013infected HeLa\u2010229 cells indicate complex host\u2010pathogen interactions at early phase of chlamydial infection.<\/jats:p>","DOI":"10.1002\/pmic.201500219","type":"journal-article","created":{"date-parts":[[2016,3,8]],"date-time":"2016-03-08T05:51:16Z","timestamp":1457416276000},"page":"1347-1360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Temporal proteomic profiling of <i>Chlamydia trachomatis<\/i>\u2013infected HeLa\u2010229 human cervical epithelial cells"],"prefix":"10.1002","volume":"16","author":[{"given":"Grace Min Yi","family":"Tan","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui Jing","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tee Cian","family":"Yeow","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaheh","family":"Movahed","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung Yeng","family":"Looi","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishein","family":"Gupta","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infection Genomics South Texas Center For Emerging Infectious Diseases University of Texas at San Antonio  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard P.","family":"Arulanandam","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infection Genomics South Texas Center For Emerging Infectious Diseases University of Texas at San Antonio  Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sazaly","family":"Abu Bakar","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Negar Shafiei","family":"Sabet","sequence":"additional","affiliation":[{"name":"Faculty of Medicine SEGi University  Petaling Jaya Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li\u2010Yen","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Won Fen","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology Tropical Infectious Disease Research and Education Centre Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/652395"},{"key":"e_1_2_8_3_1","unstructured":"Organization G. 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