{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T15:12:52Z","timestamp":1792509172857,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T00:00:00Z","timestamp":1462838400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Lundbeck Foundation Initiative for Integrative Psychiatric Research (iPSYCH), Aarhus University, Denmark, and John and Birthe Meyer Foundation"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:p>Histone modifications play an important role in regulating chromatin stability and gene expression, but to date, investigating them remains challenging. In order to obtain peptides suitable for MS\u2010based analysis, chemical derivatization of N\u2010terminus and lysine residues by propionic anhydride is commonly performed. Several side reactions (methyl\u2010esterification, amidation, solvolysis, overpropionylation, and missed propionylation) during propionylation protocols have been described, yet their relative abundances remain vague. Because methyl\u2010esterification could interfere with correct interpretation of the modification pattern, it is essential to take measures to avoid it. Here we present in\u2010depth quantitative analyses of methyl\u2010esterification and the other side reactions in a standard propionylation protocol containing methanol, and when replacing methanol with isopropanol or acetonitrile. We show that the use of alternative solvents can eliminate methyl\u2010esterification and that even though other side reactions are not prevented, their contribution can be kept relatively small. We also show that replacing methanol can be of importance also in other proteomics methods, such as mixed cation exchange, using methanol under acidic conditions.<\/jats:p>","DOI":"10.1002\/pmic.201500425","type":"journal-article","created":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T07:23:17Z","timestamp":1460704997000},"page":"2059-2063","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Quantitative assessment of methyl\u2010esterification and other side reactions in a standard propionylation protocol for detection of histone modifications"],"prefix":"10.1002","volume":"16","author":[{"given":"Veerle","family":"Paternoster","sequence":"first","affiliation":[{"name":"Department of Biomedicine Aarhus University  Aarhus Denmark"},{"name":"The Lundbeck Foundation Initiative for Integrative Psychiatric Research <i>i<\/i>PSYCH, Aarhus and Copenhagen  Denmark"},{"name":"Centre for Integrative Sequencing 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