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Tissue engineering represents an attractive therapeutic approach to treating T\/L injury but it is hampered by our poor understanding of the defining characteristics of the two tissues. The present study aimed to determine differences in the proteomic profile between native T\/Ls and tissue engineered (TE) T\/L constructs. The canine long digital extensor tendon and anterior cruciate ligament were analyzed along with 3D TE fibrin\u2010based constructs created from their cells. Native tendon and ligament differed in their content of key structural proteins, with the ligament being more abundant in fibrocartilaginous proteins. 3D T\/L TE constructs contained less extracellular matrix (ECM) proteins and had a greater proportion of cellular\u2010associated proteins than native tissue, corresponding to their low collagen and high DNA content. Constructs were able to recapitulate native T\/L tissue characteristics particularly with regard to ECM proteins. However, 3D T\/L TE constructs had similar ECM and cellular protein compositions indicating that cell source may not be an important factor for T\/L tissue engineering.<\/jats:p>","DOI":"10.1002\/pmic.201500459","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T22:46:27Z","timestamp":1460673987000},"page":"1547-1556","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Proteomic differences between native and tissue\u2010engineered tendon and ligament"],"prefix":"10.1002","volume":"16","author":[{"given":"Yalda A.","family":"Kharaz","sequence":"first","affiliation":[{"name":"Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease University of Liverpool  Leahurst Campus Neston UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon R.","family":"Tew","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease University of Liverpool  Leahurst Campus Neston UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mandy","family":"Peffers","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease University of Liverpool  Leahurst Campus Neston UK"},{"name":"The MRC\u2010Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA)  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth G.","family":"Canty\u2010Laird","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease University of Liverpool  Leahurst Campus Neston UK"},{"name":"The MRC\u2010Arthritis Research UK Centre for Integrated Research into Musculoskeletal Ageing (CIMA)  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eithne","family":"Comerford","sequence":"additional","affiliation":[{"name":"Department of Musculoskeletal Biology, Institute of Ageing and Chronic Disease University of Liverpool  Leahurst Campus Neston UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.32098\/mltj.01.2013.04"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2165\/00007256-200333010-00006"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1067\/mem.2001.111707"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.2012.00563.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3415\/VCOT-10-04-0055"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0278-5919(03)00004-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0838.2009.00938.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.20456"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516409X480395"},{"key":"e_1_2_8_11_1","first-page":"917","article-title":"Anterior cruciate ligament injury: diagnosis, management, and prevention","volume":"82","author":"Cimino F.","year":"2010","journal-title":"Am. 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