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This method, at a proof of concept stage, builds upon recently published work demonstrating the opportunity to profile and identify multiple blood signatures in bloodstains via a bottom up proteomic approach. The present protocol addresses the limitation of the previously developed profiling method with respect to destructivity; destructivity should be avoided for evidence such as blood fingermarks, where the ridge detail must be preserved in order to provide the associative link between the biometric information and the events of bloodshed. Using a blood mark reference model, trypsin concentration and spraying conditions have been optimised within the technical constraints of the depositor eventually employed; the application of MALDI\u2010MSI and Ion Mobility MS have enabled the detection, confirmation and visualisation of blood signatures directly onto the ridge pattern. These results are to be considered a first insight into a method eventually informing investigations (and judicial debates) of violent crimes in which the reliable and non\u2010destructive detection and mapping of blood in fingermarks is paramount to reconstruct the events of bloodshed.<\/jats:p>","DOI":"10.1002\/pmic.201500544","type":"journal-article","created":{"date-parts":[[2016,4,30]],"date-time":"2016-04-30T11:56:42Z","timestamp":1462017402000},"page":"1707-1717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Proteomics goes forensic: Detection and mapping of blood signatures in fingermarks"],"prefix":"10.1002","volume":"16","author":[{"given":"Lisa","family":"Deininger","sequence":"first","affiliation":[{"name":"Centre for Mass Spectrometry Imaging Biomolecular Research Centre Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekta","family":"Patel","sequence":"additional","affiliation":[{"name":"Centre for Mass Spectrometry Imaging Biomolecular Research Centre Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malcolm R.","family":"Clench","sequence":"additional","affiliation":[{"name":"Centre for Mass Spectrometry Imaging Biomolecular Research Centre Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vaughn","family":"Sears","sequence":"additional","affiliation":[{"name":"Centre for Applied Science and Technology Home Office  St Albans UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Sammon","sequence":"additional","affiliation":[{"name":"Materials and Engineering Research Institute Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Francese","sequence":"additional","affiliation":[{"name":"Centre for Mass Spectrometry Imaging Biomolecular Research Centre Sheffield Hallam University  Sheffield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3an36896c"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-015-1140-z"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep11716"},{"key":"e_1_2_8_5_1","first-page":"1","volume-title":"Fingermark Visualization Manual","author":"Bleay S. 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