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In particular, neural cells such as neurons and astrocytes are known to express unique functional glycans not found in other cells, and these glycans play critical roles in high\u2010order brain functions and various neurological disorders. However, little is known about how the expression of these neural glycans is established and maintained. Here, we investigated which glycans are expressed in each primary neural cell and how epigenetics contributes to the expression of neural glycans. We first isolated primary neurons, astrocytes, and fibroblasts from mouse embryos and carried out <jats:italic>N<\/jats:italic>\u2010glycomic and glycosyltransferase (GlycoT)\u2010transcriptomic analyses to identify <jats:italic>N<\/jats:italic>\u2010glycans specific to a particular neural cell type and to clarify the underlying transcriptional basis. We next treated the cells with epigenetic drugs (5\u2010aza\u20102\u2032\u2010deoxycytidine (5\u2010aza) and trichostatin A (TSA)) and characterized the changes in GlycoT\u2010transcriptomes and <jats:italic>N<\/jats:italic>\u2010glycomes. We found that the <jats:italic>N<\/jats:italic>\u2010glycomes in neurons were highly stable and resistant to epigenetic stimulation. In contrast, astrocytes showed dynamic <jats:italic>N<\/jats:italic>\u2010glycan changes after treatment, such as a shift in the linkages of sialic acid. These results provide novel insights into how the expression of neural glycans is maintained and epigenetically regulated.<\/jats:p>","DOI":"10.1002\/pmic.201600053","type":"journal-article","created":{"date-parts":[[2016,6,11]],"date-time":"2016-06-11T03:59:57Z","timestamp":1465617597000},"page":"2854-2863","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Epigenetic regulation of neural <i>N<\/i>\u2010glycomics"],"prefix":"10.1002","volume":"16","author":[{"given":"Yasuhiko","family":"Kizuka","sequence":"first","affiliation":[{"name":"Disease Glycomics Team, Systems Glycobiology Research Group, RIKEN\u2010Max Planck Joint Research Center for Systems Chemical Biology, Global Research Cluster RIKEN  Wako Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyako","family":"Nakano","sequence":"additional","affiliation":[{"name":"Graduate School of Advanced Sciences of Matter Hiroshima University  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