{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:33:58Z","timestamp":1792712038081,"version":"4.5.0"},"reference-count":65,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2016,12,6]],"date-time":"2016-12-06T00:00:00Z","timestamp":1480982400000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006528","name":"Alzheimer's Disease Research Center, Emory University","doi-asserted-by":"publisher","award":["P50AG025688"],"award-info":[{"award-number":["P50AG025688"]}],"id":[{"id":"10.13039\/100006528","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000065","name":"National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["K08NS087121"],"award-info":[{"award-number":["K08NS087121"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000957","name":"Alzheimer's Association","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000957","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002283","name":"Alzheimer\u2019s Research UK","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006312","name":"BrightFocus Foundation","doi-asserted-by":"publisher","award":["A2015332S"],"award-info":[{"award-number":["A2015332S"]}],"id":[{"id":"10.13039\/100006312","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P50AG025688"],"award-info":[{"award-number":["P50AG025688"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["U01AG046161\u201002"],"award-info":[{"award-number":["U01AG046161\u201002"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:p>Despite a key role of amyloid\u2010beta (A\u03b2) in Alzheimer's disease (AD), mechanisms that link A\u03b2 plaques to tau neurofibrillary tangles and cognitive decline still remain poorly understood. The purpose of this study was to quantify proteins in the sarkosyl\u2010insoluble brain proteome correlated with A\u03b2 and tau insolubility in the asymptomatic phase of AD (AsymAD) and through mild cognitive impairment (MCI) and symptomatic AD. Employing label\u2010free mass spectrometry\u2010based proteomics, we quantified 2711 sarkosyl\u2010insoluble proteins across the prefrontal cortex from 35 individual cases representing control, AsymAD, MCI and AD. Significant enrichment of A\u03b2 and tau in AD was observed, which correlated with neuropathological measurements of plaque and tau tangle density, respectively. Pairwise correlation coefficients were also determined for all quantified proteins to A\u03b2 and tau, across the 35 cases. Notably, six of the ten most correlated proteins to A\u03b2 were U1 small nuclear ribonucleoproteins (U1 snRNPs). Three of these U1 snRNPs (U1A, SmD and U1\u201070K) also correlated with tau consistent with their association with tangle pathology in AD. Thus, proteins that cross\u2010correlate with both A\u03b2 and tau, including specific U1 snRNPs, may have potential mechanistic roles in linking A\u03b2 plaques to tau tangle pathology during AD progression.<\/jats:p>","DOI":"10.1002\/pmic.201600057","type":"journal-article","created":{"date-parts":[[2016,10,13]],"date-time":"2016-10-13T02:45:24Z","timestamp":1476326724000},"page":"3042-3053","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":103,"title":["Changes in the detergent\u2010insoluble brain proteome linked to amyloid and tau in Alzheimer's Disease progression"],"prefix":"10.1002","volume":"16","author":[{"given":"Chadwick M.","family":"Hales","sequence":"first","affiliation":[{"name":"Department of Neurology Emory University School of Medicine  Atlanta GA USA"},{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric B.","family":"Dammer","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiudong","family":"Deng","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duc M.","family":"Duong","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marla","family":"Gearing","sequence":"additional","affiliation":[{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"},{"name":"Department of Experimental Pathology Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Troncoso","sequence":"additional","affiliation":[{"name":"Departments of Pathology and Neurology Johns Hopkins School of Medicine  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhav","family":"Thambisetty","sequence":"additional","affiliation":[{"name":"National Institute on Aging National Institutes of Health  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James J.","family":"Lah","sequence":"additional","affiliation":[{"name":"Department of Neurology Emory University School of Medicine  Atlanta GA USA"},{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua M.","family":"Shulman","sequence":"additional","affiliation":[{"name":"Departments of Neurology Neuroscience and Molecular &amp; Human Genetics and Program in Developmental Biology, Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston Baylor College of Medicine  Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allan I.","family":"Levey","sequence":"additional","affiliation":[{"name":"Department of Neurology Emory University School of Medicine  Atlanta GA USA"},{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas T.","family":"Seyfried","sequence":"additional","affiliation":[{"name":"Department of Neurology Emory University School of Medicine  Atlanta GA USA"},{"name":"Center for Neurodegenerative Disease Emory University School of Medicine  Atlanta GA USA"},{"name":"Department of Biochemistry Emory University School of Medicine  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1067122"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1066"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006189"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-6147(91)90609-V"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.1995.00540250089017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jalz.2011.03.003"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072994"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2009-1179"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1310249110"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900474t"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.41.4.479"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00308809"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cercor\/bhn113"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.562959"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400246t"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400189"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr2010814"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1019"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr900251d"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/prca.201100068"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/alzrt186"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr2010814"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M500230-MCP200"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts366"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(95)11484-E"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12133"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.ajp.159.5.738"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2014.07.001"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1091230"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-200012000-00025"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3040-05.2005"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141448"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v046.i11"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-0631-0_86"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0084547"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3505"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.316232"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1402645111"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90941-4"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.1389"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02890024"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-9-15"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/S1474-4422(13)70090-5","article-title":"Tau pathology and neurodegeneration","volume":"12","author":"Spillantini M. G.","journal-title":"Lancet Neurol."},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2006.71.001"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1109434108"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11922"},{"key":"e_1_2_8_49_1","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.neuron.2015.10.030","article-title":"ALS\/FTD mutation\u2010induced phase transition of FUS liquid droplets and reversible hydrogels into irreversible hydrogels impairs RNP granule function","volume":"88","author":"Murakami T.","journal-title":"Neuron"},{"key":"e_1_2_8_50_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.cell.2015.09.015","article-title":"Phase separation by low complexity domains promotes stress granule assembly and drives pathological fibrillization","volume":"163","author":"Molliex A.","journal-title":"Cell"},{"key":"e_1_2_8_51_1","doi-asserted-by":"crossref","first-page":"1455","DOI":"10.1016\/j.celrep.2016.04.045","article-title":"Interaction of tau with the RNA\u2010Binding Protein tia1 regulates tau pathophysiology and toxicity","volume":"15","author":"Vanderweyde T.","journal-title":"Cell Reports"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddt478"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv107"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2681-12.2012"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4299"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/NEN.0000000000000181"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2013.00015"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2016.03.019"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2542-13.2013"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2014.11.019"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1860-14.2014"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad8373"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-9158-3-23"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0038658"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200006"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3700"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201600057","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201600057","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201600057","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T10:38:24Z","timestamp":1761043104000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201600057"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,12]]},"references-count":65,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2016,12]]}},"alternative-id":["10.1002\/pmic.201600057"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201600057","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,12]]},"assertion":[{"value":"2016-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}