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This provides strong impetus to push for early GC detection through the use of non\u2010invasive biomarkers, before metastatic complications arise. It is also of strong medical interest to identify patients of the diffuse subtype at the earliest time possible, since the disease variant progresses very rapidly and is associated with much higher mortality rate. In this study, we compared quantitatively the gastric fluid proteome of 70 GC patients to 17 individuals with benign gastritis in search of potential biomarkers that aid in GC diagnosis, prognosis and subtype stratification. We report that as much as half of the gastric fluid proteome is subject to regulation in diseased states, and propose a simple biomarker panel scoring matrix for early GC detection with diagnostic sensitivity of 95.7%. We also demonstrate as proof\u2010of\u2010concept that a digitised record generated with SWATH\u2010MS based on 380 protein abundance signatures from the gastric fluid could segregate patients with diffuse\u2010type GC.<\/jats:p>","DOI":"10.1002\/pmic.201600194","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T05:22:32Z","timestamp":1470288152000},"page":"2921-2930","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A simple biomarker scoring matrix for early gastric cancer detection"],"prefix":"10.1002","volume":"16","author":[{"given":"Wei","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen Wei","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore  Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maxey C. 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