{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:39:06Z","timestamp":1775767146389,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2017,10,25]],"date-time":"2017-10-25T00:00:00Z","timestamp":1508889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273484"],"award-info":[{"award-number":["81273484"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Triptolide (TP), the major active component in\n                    <jats:italic>Tripterygium wilfordii<\/jats:italic>\n                    Hook. f., is widely used for the treatment of rheumatoid arthritis and autoimmune diseases. However, organ toxicity, especially hepatotoxicity and nephrotoxicity, limits its clinical application. To fully understand the mechanism underlying TP toxicity, iTRAQ\u2010based 2D\u2010LC\u2010MS\/MS proteomics is used to detect differentially expressed proteins in the livers and kidneys of mice administered the LD50 dose of TP. Functional annotation revealed that multiple pathways are involved in TP toxicity, including acute\u2010phase response signaling, the antigen presentation pathway, FXR\/RXR activation, LPS\/IL\u20101\u2010mediated inhibition of RXR function, and EIF2 signaling.\u00a0Members of the cytochrome\u00a0P450 protein family that are involved in fatty acid (FA) metabolism, such as CYP2E1, show significant differences in expression among groups. Additionally, the proteomics data suggested that FAs are involved in TP\u2010induced toxicity. FA analysis is conducted using HPLC\u2010MRM to characterize the differences among various groups exposed to TP for different times. It has been found that 20 FAs in the liver show significant differences in abundance among groups, whereas in the kidneys, six FAs show significant differences in abundance. By integrating the proteomic and targeted FA analyses, it has been found that differently expressed proteins and FAs both participate in pathways including cellular lipolytic activity, peroxisomal fatty acid beta\u2010oxidation, and so on. Our data contribute to understanding the mechanisms underlying TP\u2010induced organ toxicity. The results may help to improve the clinical efficacy and safety of TP in the future.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201700001","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T05:24:43Z","timestamp":1505193883000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Quantitative Proteomics and Targeted Fatty Acids Analysis Reveal the Damage of Triptolide in Liver and Kidney"],"prefix":"10.1002","volume":"17","author":[{"given":"Menglin","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cai","family":"Tie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Qu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing P. R. 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