{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:26:15Z","timestamp":1782815175259,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T00:00:00Z","timestamp":1490918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:p>Extended spectrum beta\u2010lactamase producing <jats:italic>Klebsiella pneumoniae<\/jats:italic> (ESBL\u2010KP) causes life\u2010threatening infections in susceptible and immuno\u2010compromised individuals. Because of the emergence of multidrug resistance and tolerance, it is crucial to better understand the mechanisms by which ESBL\u2010KP can adapt to antibiotic stress. The aim of this study was to provide an overview of the global proteome changes occurring in ESBL\u2010KP in response to sub\u2010lethal concentrations of the antibiotics doxycycline (DC, bacteriostatic) and streptomycin (SM, bactericidal), which both impair ribosomal synthesis of bacterial proteins. These results represent the greatest experimental coverage of the ESBL\u2010KP proteome yet described. The 1538 proteins, representing 30% of the 5126 predicted KP gene products were identified from the combined experimental groups. Antibiotic stress resulted in significantly elevated levels of 42 proteins for DC and 55 for SM treatments, whereas 53 proteins were reduced for DC\u2010 and six for SM\u2010treated bacteria. Specifically, the ESBL\u2010KP response to DC was accompanied by the reduced levels of the porins LamB, CirA, FepA, and OmpC. In contrast to DC, the stress response to SM demonstrated a dramatic increase in the peroxidase detoxification pathway proteins PutA, KatG, KatE, and Dps, which prevent harmful hydroxyl radical formation. The results from this proteomic study are important for understanding adaptive responses to antibiotics, and may provide novel targets for the development of new therapeutic strategies.<\/jats:p>","DOI":"10.1002\/pmic.201700003","type":"journal-article","created":{"date-parts":[[2017,2,15]],"date-time":"2017-02-15T03:52:44Z","timestamp":1487130764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Antibiotic\u2010dependent perturbations of extended spectrum beta\u2010lactamase producing <i>Klebsiella pneumoniae<\/i> proteome"],"prefix":"10.1002","volume":"17","author":[{"given":"Moo\u2010Jin","family":"Suh","sequence":"first","affiliation":[{"name":"Molecular and Translational Sciences Division U.S. Army Medical Research Institute of Infectious Diseases  Frederick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Keasey","sequence":"additional","affiliation":[{"name":"Molecular and Translational Sciences Division U.S. Army Medical Research Institute of Infectious Diseases  Frederick MD USA"},{"name":"Biological Sciences Department University of Maryland Baltimore County  Baltimore MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst E.","family":"Brueggemann","sequence":"additional","affiliation":[{"name":"Molecular and Translational Sciences Division U.S. Army Medical Research Institute of Infectious Diseases  Frederick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert G.","family":"Ulrich","sequence":"additional","affiliation":[{"name":"Molecular and Translational Sciences Division U.S. Army Medical Research Institute of Infectious Diseases  Frederick MD USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,3,31]]},"reference":[{"key":"e_1_2_4_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1870-y"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00285-06"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp327"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1306801"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1322"},{"key":"e_1_2_4_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.261"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1511"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr101065j"},{"key":"e_1_2_4_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1994"},{"key":"e_1_2_4_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.5.751"},{"key":"e_1_2_4_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.10.3332-3335.2003"},{"key":"e_1_2_4_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01441-10"},{"key":"e_1_2_4_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr100740w"},{"key":"e_1_2_4_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2007.10.003"},{"key":"e_1_2_4_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2012.10.001"},{"key":"e_1_2_4_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb742"},{"key":"e_1_2_4_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M412706200"},{"key":"e_1_2_4_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.06.049"},{"key":"e_1_2_4_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203977200"},{"key":"e_1_2_4_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp964"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201700003","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201700003","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T12:47:43Z","timestamp":1761050863000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201700003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,31]]},"references-count":20,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1002\/pmic.201700003"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201700003","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3,31]]},"assertion":[{"value":"2017-01-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-03-31","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1700003"}}