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Despite their importance in mounting an immune response, an unbiased description of proteins expressed by chicken lymphocytes has not been presented. In this study, we therefore intravenously infected chickens with <jats:italic>Salmonella<\/jats:italic> Enteritidis, sorted CD4, CD8 and \u03b3\u03b4 T\u2010lymphocytes from the spleen by flow cytometry and determined the proteome of each population by LC\u2010MS\/MS. CD4 T\u2010lymphocyte characteristic proteins included ubiquitin SUMO\u2010like domain and BAR domain containing proteins. CD8 T\u2010lymphocyte specific proteins were characterized by purine ribonucleoside triphosphate binding and were involved in cell differentiation, cell activation and regulation of programmed cell death. \u03b3\u03b4 T\u2010lymphocyte specific proteins exhibited enrichment of small GTPase of Rab type and GTP binding. Following infection, inducible proteins in CD4 lymphocytes included ribosomal proteins and downregulated proteins localized to the lysosome. CD8 T\u2010lymphocytes induced MCM complex proteins, proteins required for DNA replication and machinery for protein processing in the endoplasmic reticulum. Proteins inducible in \u03b3\u03b4 T\u2010lymphocytes belonged to immune system response, oxidative phosphorylation and the spliceosome. In this study, we predicted the likely events in lymphocyte response to systemic bacterial infection and identified proteins which can be used as markers specific for each lymphocyte subpopulation.<\/jats:p>","DOI":"10.1002\/pmic.201700073","type":"journal-article","created":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T10:52:45Z","timestamp":1497610365000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Different roles of CD4, CD8 and \u03b3\u03b4 T\u2010lymphocytes in naive and vaccinated chickens during <i>Salmonella<\/i> Enteritidis infection"],"prefix":"10.1002","volume":"17","author":[{"given":"Zuzana","family":"Sekelova","sequence":"first","affiliation":[{"name":"Veterinary Research Institute  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Polansky","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute  Brno Czech 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