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Using <jats:italic>Salmonella<\/jats:italic> infection of host epithelial cells as a model system, we previously analyzed intracellular bacterial proteome as a window into pathogens\u2019 adaptations to their host environment [<jats:italic>Infect. Immun<\/jats:italic>. 2015; <jats:italic>J. Proteome Res<\/jats:italic>. 2017]. Herein we extended our efforts to quantitatively examine protein expression of host cells during infection. In total, we identified more than 5000 proteins with 194 differentially regulated proteins upon bacterial infection. Notably, we found marked induction of host integrin signaling and glycolytic pathways. Intriguingly, up\u2010regulation of host glucose metabolism concurred with increased utilization of glycolysis by intracellular <jats:italic>Salmonella<\/jats:italic> during infection. In addition to immunoblotting assays, we also verified the up\u2010regulation of PARP1 in the host nucleus by selected reaction monitoring and immunofluorescence studies. Furthermore, we provide evidence that PARP1 elevation is likely specific to <jats:italic>Salmonella<\/jats:italic> infection and independent of one of the bacterial type III secretion systems. Our work demonstrates that unbiased high\u2010throughput proteomics can be used as a powerful approach to provide new perspectives on host\u2013pathogen interactions.<\/jats:p>","DOI":"10.1002\/pmic.201700092","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T13:20:47Z","timestamp":1495718447000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Quantitative proteomic analysis of host epithelial cells infected by <i>Salmonella enterica<\/i> serovar Typhimurium"],"prefix":"10.1002","volume":"17","author":[{"given":"Linlu","family":"Qi","sequence":"first","affiliation":[{"name":"Institute of Analytical Chemistry and Synthetic and Functional Biomolecules Center, College of Chemistry and Molecular Engineering Peking University  Beijing P. R. 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