{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T02:43:38Z","timestamp":1787798618473,"version":"build-2784847793"},"reference-count":17,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2017,10,16]],"date-time":"2017-10-16T00:00:00Z","timestamp":1508112000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Programme Orientations Strat\u00e9giques from the University of Lille 2"},{"name":"Centre National de la Recherche Scientifique and the Institut Pasteur of Lille"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. is known for years as a highly prevalent anaerobic eukaryotic parasite of humans and animals. Several monophyletic clades have been delineated based on molecular data, and the occurrence of each subtype in humans and\/or animal hosts has been documented. The genome of several representatives has been sequenced revealing specific traits such as an intriguing 3'\u2010end processing of primary transcripts. Here, a first high\u2010throughput proteomics dataset acquired on this difficult\u2010to\u2010cultivate parasite is presented for the zoonotic subtype T4 isolate WR1. Amongst the 2766 detected proteins, we highlighted the role of a small ADP ribosylation factor GTP\u2010binding protein involved in intracellular traffic as major regulator of vesicle biogenesis and a voltage\u2010dependent anion\u2010selective channel protein because both were unexpectedly highly abundant. We show how these data may be used for gaining proteogenomics insights into\n                    <jats:italic>Blastocystis<\/jats:italic>\n                    sp. specific molecular mechanisms. We evidenced for the first time by proteogenomics a functional termination codon derived from transcript polyadenylation for seven different key cellular components.\n                  <\/jats:p>","DOI":"10.1002\/pmic.201700211","type":"journal-article","created":{"date-parts":[[2017,9,4]],"date-time":"2017-09-04T12:22:43Z","timestamp":1504527763000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteogenomic Insights into the Intestinal Parasite\n                    <i>Blastocystis<\/i>\n                    sp. Subtype 4 Isolate WR1"],"prefix":"10.1002","volume":"17","author":[{"given":"Jean","family":"Armengaud","sequence":"first","affiliation":[{"name":"Laboratoire Innovations technologiques pour la D\u00e9tection et le Diagnostic (Li2D) Service de Pharmacologie et Immunoanalyse (SPI) CEA, INRA  Gif\u2010Sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Pible","sequence":"additional","affiliation":[{"name":"Laboratoire Innovations technologiques pour la D\u00e9tection et le Diagnostic (Li2D) Service de Pharmacologie et Immunoanalyse (SPI) CEA, INRA  Gif\u2010Sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Charles","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Laboratoire Innovations technologiques pour la D\u00e9tection et le Diagnostic (Li2D) Service de Pharmacologie et Immunoanalyse (SPI) CEA, INRA  Gif\u2010Sur\u2010Yvette France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amandine","family":"Cian","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille CNRS, Inserm, CHU Lille Institut Pasteur de Lille U1019\u2013UMR 8204\u2013CIIL\u2013Centre d'Infection et d'Immunit\u00e9 de Lille 1 rue du Professeur Calmette, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nausicaa","family":"Gantois","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille CNRS, Inserm, CHU Lille Institut Pasteur de Lille U1019\u2013UMR 8204\u2013CIIL\u2013Centre d'Infection et d'Immunit\u00e9 de Lille 1 rue du Professeur Calmette, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin S. W.","family":"Tan","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Parasitology Department of Microbiology and Immunology Yong Loo Lin School of Medicine National University of Singapore  Singapore Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magali","family":"Chab\u00e9","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille CNRS, Inserm, CHU Lille Institut Pasteur de Lille U1019\u2013UMR 8204\u2013CIIL\u2013Centre d'Infection et d'Immunit\u00e9 de Lille 1 rue du Professeur Calmette, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Viscogliosi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lille CNRS, Inserm, CHU Lille Institut Pasteur de Lille U1019\u2013UMR 8204\u2013CIIL\u2013Centre d'Infection et d'Immunit\u00e9 de Lille 1 rue du Professeur Calmette, Lille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2016.05.007"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2016.05.015"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2013.05.003"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2016.03.017"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002885"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-3-r29"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.02.003"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gdata.2015.01.009"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2016.02.025"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-1203-1"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1261-2_16"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.013102"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/17435390.2016.1244299"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.86"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201400157"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/gbe\/evu146"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv1145"}],"container-title":["PROTEOMICS"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpmic.201700211","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pmic.201700211","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T18:03:27Z","timestamp":1761069807000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/pmic.201700211"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,16]]},"references-count":17,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/pmic.201700211"],"URL":"https:\/\/doi.org\/10.1002\/pmic.201700211","archive":["Portico"],"relation":{},"ISSN":["1615-9853","1615-9861"],"issn-type":[{"value":"1615-9853","type":"print"},{"value":"1615-9861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,16]]},"assertion":[{"value":"2017-05-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1700211"}}