{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:43:31Z","timestamp":1770349411684,"version":"3.49.0"},"reference-count":75,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2018,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bordetella pertussis<\/jats:italic> causes whooping cough. The predominant strains in Australia changed to single nucleotide polymorphism (SNP) cluster I (pertussis toxin promoter allele <jats:italic>ptxP3\/<\/jats:italic>pertactin gene allele <jats:italic>prn2<\/jats:italic>) from cluster II (non\u2010<jats:italic>ptxP3<\/jats:italic>\/non\u2010<jats:italic>prn2<\/jats:italic>). Cluster I was mostly responsible for the 2008\u20132012 Australian epidemic and was found to have higher fitness compared to cluster II using an <jats:italic>in vivo<\/jats:italic> mouse competition assay, regardless of host's immunization status. This study aimed to identify proteomic differences that explain higher fitness in cluster I using isobaric tags for relative and absolute quantification (iTRAQ), and high\u2010resolution multiple reaction monitoring (MRM\u2010hr). A few key differences in the whole cell and secretome were identified between the cluster I and II strains tested. In the whole cell, nine proteins were upregulated (&gt;1.2 fold change, <jats:italic>q<\/jats:italic> &lt; 0.05) and three were downregulated (&lt;0.8 fold change, <jats:italic>q<\/jats:italic> &lt; 0.05) in cluster I. One downregulated protein was BP1569, a TLR2 agonist for Th1 immunity. In the secretome, 12 proteins were upregulated and 1 was downregulated which was Bsp22, a type III secretion system (T3SS) protein. Furthermore, there was a trend of downregulation in three T3SS effectors and other virulence factors. Three proteins were upregulated in both whole cell and supernatant: BP0200, molybdate ABC transporter (ModB), and tracheal colonization factor A (TcfA). Important expression differences in lipoprotein, T3SS, and transport proteins between the cluster I and II strains were identified. These differences may affect immune evasion, virulence and metabolism, and play a role in increased fitness of cluster I.<\/jats:p>","DOI":"10.1002\/pmic.201700237","type":"journal-article","created":{"date-parts":[[2018,2,21]],"date-time":"2018-02-21T15:26:41Z","timestamp":1519226801000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Proteomic Adaptation of Australian Epidemic <i>Bordetella pertussis<\/i>"],"prefix":"10.1002","volume":"18","author":[{"given":"Laurence Don Wai","family":"Luu","sequence":"first","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophie","family":"Octavia","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Zhong","sequence":"additional","affiliation":[{"name":"Mark Wainwright Analytical Centre University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark J.","family":"Raftery","sequence":"additional","affiliation":[{"name":"Mark Wainwright Analytical Centre University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vitali","family":"Sintchenko","sequence":"additional","affiliation":[{"name":"Centre for Infectious Diseases and Microbiology\u2013Public Health Institute of Clinical Pathology and Medical Research\u2013Pathology West Westmead Hospital  New South Wales Australia"},{"name":"Marie Bashir Institute for Infectious Diseases and Biosecurity Sydney Medical School University of Sydney  New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruiting","family":"Lan","sequence":"additional","affiliation":[{"name":"School of Biotechnology and Biomolecular Sciences University of New South Wales  Sydney New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1016\/j.vaccine.2010.11.059","volume":"29","author":"Publication W.","year":"2011","journal-title":"Vaccine"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00669-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpc.12796"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","first-page":"297","DOI":"10.3201\/eid1602.081707","volume":"16","author":"Kurniawan J.","year":"2010","journal-title":"Emerg. iInfect. 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