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Female C57Bl\/6 mice were randomized into three groups: Control, Limited Food Access (LFA) and Activity\u2010Based Anorexia (ABA). LFA and ABA mice had a progressive limited access to food but only ABA mice had access to an activity wheel. On colonic mucosal protein extracts, a 2D PAGE\u2010based comparative proteomic analysis was then performed and differentially expressed proteins were identified by LC\u2010ESI\u2010MS\/MS. Twenty\u2010seven nonredundant proteins that were differentially expressed between Control, LFA, and ABA groups were identified. ABA mice exhibited alteration of several mitochondrial proteins involved in energy metabolism such as dihydrolipoyl dehydrogenase and 3\u2010mercaptopyruvate sulfurtransferase. In addition, a downregulation of mammalian target of rapamycin (mTOR) pathway was observed leading, on the one hand, to the inhibition of protein synthesis, evaluated by puromycin incorporation and mediated by the increased phosphorylation of eukaryotic elongation factor 2, and on the other hand, to the activation of autophagy, assessed by the increase of the marker of autophagy, form LC3\u2010phosphatidylethanolamine conjugate\/Cytosolic form of Microtubule\u2010associated protein 1A\/1B light chain 3 (LC3II\/LC3I) ratio. Colonic mucosal proteome is altered during ABA suggesting a downregulation of energy metabolism. A decrease of protein synthesis and an activation of autophagy were also observed mediated by mTOR pathway.<\/jats:p>","DOI":"10.1002\/pmic.201700395","type":"journal-article","created":{"date-parts":[[2018,6,25]],"date-time":"2018-06-25T06:53:43Z","timestamp":1529909623000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Colonic Mucosal Proteome Signature Reveals Reduced Energy Metabolism and Protein Synthesis but Activated Autophagy during Anorexia\u2010Induced Malnutrition in Mice"],"prefix":"10.1002","volume":"18","author":[{"given":"S\u00e9verine","family":"Nobis","sequence":"first","affiliation":[{"name":"INSERM Unit 1073 UNIROUEN Normandie University  76000 Rouen France"},{"name":"Institute for Research and Innovation in Biomedicine UNIROUEN Normandie University  76000 Rouen 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