{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T11:21:06Z","timestamp":1788175266378,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,27]],"date-time":"2018-12-27T00:00:00Z","timestamp":1545868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Proteomics"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exosomes are typically involved in cellular communication and signaling. Macrophages play a key role in lipopolysaccharide (LPS)\u2010induced sepsis. However, the molecular comparison of exosomes derived from LPS\u2010induced macrophage has not been well analyzed. The macrophage\u2010exosomes are validated and the protein composition of those exosomes are investigated by isobaric tags for relative and absolute quantification (iTRAQ) mass spectrometry. A total of 5056 proteins are identified in macrophage\u2010exosomes. We discovered 341 increased proteins and 363 reduced proteins in LPS\u2010treated macrophage\u2010exosomes compared with control exosomes. In addition, gene ontology analysis demonstrates that macrophage\u2010exosomes proteins are mostly linked to cell, organelle, extracellular region, and membrane. The bioinformatics analysis also indicates that these proteins are mainly involved in cellular process, single\u2010organism process, metabolic process, and biological regulation. Among these 341 upregulated proteins, Kyoto Encyclopedia of Genes and Genomes analysis reveals that 22 proteins are involved in the NOD\u2010like receptor signaling pathway. Finally, hepatocytes can uptake macrophage\u2010exosomes and subsequently NLRP3 inflammasome is activated in vitro and in vivo. These data emphasize the fundamental importance of macrophage\u2010exosomes in sepsis\u2010induced liver injury. Therefore, the iTRAQ proteomic strategy brings new insights into macrophage\u2010derived exosomes. It may improve our understanding of macrophage\u2010exosomes\u2019 functions and their possible use as therapeutic targets for sepsis.<\/jats:p>","DOI":"10.1002\/pmic.201800274","type":"journal-article","created":{"date-parts":[[2018,11,26]],"date-time":"2018-11-26T08:15:18Z","timestamp":1543220118000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Proteomic Profiling of LPS\u2010Induced Macrophage\u2010Derived Exosomes Indicates Their Involvement in Acute Liver Injury"],"prefix":"10.1002","volume":"19","author":[{"given":"Guozhen","family":"Wang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University  Guangzhou 510515 China"},{"name":"State Key Laboratory of Organ Failure Research Department of Emergency Medicine Guangdong Provincial Key Laboratory of Viral Hepatitis Research Nanfang Hospital 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